Department of Hematology, Renji Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai, People's Republic of China.
Int J Hematol. 2010 Jul;92(1):129-35. doi: 10.1007/s12185-010-0626-5. Epub 2010 Jun 25.
The expression pattern of microRNAs (miRNAs) and their potential target genes were investigated in acute promyelocytic leukemia (APL) cell line NB4 cells during all-trans-retinoid acid (ATRA) treatment by using a miRNA microarrays platform and real-time quantitative PCR (RTQ-PCR). MiR-146a as one of the miRNAs down-regulated by ATRA during APL differentiation was identified. Direct interaction between miR146a and its predictive target gene Smad4 were confirmed by Luciferase assay. Down-regulation of miR-146a and upregulation of Smad4 at protein levels were demonstrated. These data suggested that miR-146a might influence proliferation of APL cells through TGF-beta1/Smad signal transduction pathway during ATRA induction.
采用 miRNA 微阵列平台和实时定量 PCR（RTQ-PCR）技术，研究了全反式维甲酸（ATRA）治疗急性早幼粒细胞白血病（APL）细胞系 NB4 细胞过程中 microRNAs（miRNAs）的表达模式及其潜在靶基因。鉴定出 miR-146a 是 ATRA 诱导 APL 分化过程中下调的 miRNAs 之一。通过荧光素酶测定证实了 miR146a 与其预测靶基因 Smad4 之间的直接相互作用。证实了 miR-146a 在蛋白质水平下调和 Smad4 上调。这些数据表明，miR-146a 可能通过 ATRA 诱导时 TGF-β1/Smad 信号转导通路影响 APL 细胞的增殖。
