[建立手术标本原位移植构建高转移胃淋巴瘤裸鼠模型]

[Establishment of a nude mouse model of highly metastatic gastric lymphoma constructed with orthotopic transplantation of surgical specimen].

作者信息

Yang Bo, Tuo Shuai, Tuo Chao-wei, Zhang Ning, Liu Qiu-zhen

机构信息

Department of Ultrapathology, No.202 Hospital of PLA, Shenyang 110003, China.

出版信息

Zhonghua Wei Chang Wai Ke Za Zhi. 2010 Jun;13(6):436-9.

PMID:20577924

Abstract

OBJECTIVE

To construct a mouse model of highly metastatic gastric lymphoma with orthotopic transplantation of human primary gastric lymphoma specimen.

METHODS

A fresh surgical specimen of primary gastric lymphoma was obtained intraoperatively and implanted into the submucosa of stomach in nude mice. Tumor formation, invasion, metastasis, morphological characteristics under light microscopy and electron microscopy, immunohistochemistry,and the karyotype of orthotopically transplanted tumor cells were studied.

RESULTS

An orthotopic highly metastatic model of human primary gastric lymphoma in nude mice(HGBL-0305) was successfully established. Histopathology of transplanted tumors showed primary gastric diffuse large B cell lymphoma. CD19, CD20, CD22 and CD79alpha were positive, while CD3 and CD7 were negative. The number of chromosome ranged from 56 to 69. DNA index(DI) was 1.47+/-0.12(i.e. heteroploid). Until now, HGBL-0305 model has been maintained for 45 generations by orthotopic passage for almost 4 years in nude mice. A total of 156 nude mice were used for transplantation. The growth rate and resuscitation rate of liquid nitrogen cryopreservation of transplanted tumor cells were both 100%. The autonomic growth of the transplanted tumor cells invaded and destructed all the layers of the nude mice stomach. The metastasis rates of liver, spleen, lymph node, and peritoneal seeding were 69.5%, 55.6%, 45.7%, and 30.5%, respectively.

CONCLUSIONS

An orthotopic highly metastatic model of human primary gastric lymphoma in nude mice is successfully established. HGBL-0305 model may simulate the natural course of primary gastric lymphoma in human and provides an ideal animal model for studies on pathogenesis, metastasis biology and anti-metastatic therapies of primary gastric lymphoma.

摘要

目的

通过原位移植人原发性胃淋巴瘤标本构建高转移性胃淋巴瘤小鼠模型。

方法

术中获取原发性胃淋巴瘤新鲜手术标本，植入裸鼠胃黏膜下层。研究肿瘤形成、侵袭、转移、光镜和电镜下的形态特征、免疫组化以及原位移植肿瘤细胞的核型。

结果

成功建立了裸鼠人原发性胃淋巴瘤原位高转移模型（HGBL-0305）。移植瘤组织病理学显示为原发性胃弥漫性大B细胞淋巴瘤。CD19、CD20、CD22和CD79α呈阳性，而CD3和CD7呈阴性。染色体数目为56至69条。DNA指数（DI）为1.47±0.12（即异倍体）。迄今为止，HGBL-0305模型已通过原位传代在裸鼠体内维持了45代，近4年。共使用156只裸鼠进行移植。移植瘤细胞液氮冻存后的生长率和复苏率均为100%。移植瘤细胞自主生长，侵袭并破坏了裸鼠胃的各层组织。肝、脾、淋巴结和腹腔种植的转移率分别为69.5%、55.6%、45.7%和30.5%。