Laboratory Bionano, Université Montpellier Sud de France, Montpellier, France.
Dent Mater. 2010 Sep;26(9):881-90. doi: 10.1016/j.dental.2010.05.003. Epub 2010 Jun 30.
Our aim was to improve the comprehension of in vitro tracer leakage studies and to determine in which conditions such studies can be reliable. We aimed to develop different theoretical models to describe either an initially dry or a wet interface (slit) between sealer and dentin.
Equations based on physical laws were derived to model theoretically in vitro tracer penetration. For the dry interfaces, atmospheric, hydrostatic, tracer gravimetric, capillary and internal air pressures were considered as the underlying forces that control tracer penetration. For wet interfaces, the laws of diffusion were used to model colorant penetration.
In both cases penetration is influenced by the width of the interface and by the size of the colorant. Calculations for dry conditions have shown that penetration is quick, mainly driven by the capillary pressure, and the penetration increases as the width of the interface diminishes. Dentinal tubules and the extent of their interconnection modify the penetration depth. For wet conditions, tracer size is the main factor controlling the penetration length and speed (the bigger the tracer, the slower the penetration).
Our model calculations demonstrate that tracer penetration studies have to be performed under strict experimental conditions. Dry and wet interfaces are two extreme cases with very different tracer penetration modes. In vitro colorant penetration tests should be performed in both of these conditions avoiding cases where the slit contains both air and water. Theses models can be adapted to other dental situations as well.
本研究旨在提高对体外示踪剂渗漏研究的理解,并确定在何种条件下这些研究具有可靠性。我们旨在开发不同的理论模型来描述密封剂和牙本质之间最初干燥或湿润的界面(缝隙)。
根据物理定律推导出了用于模拟体外示踪剂渗透的理论方程。对于干燥界面,考虑大气压力、静水压力、示踪剂重量、毛细压力和内部空气压力作为控制示踪剂渗透的潜在力。对于湿润界面,使用扩散定律来模拟着色剂的渗透。
在这两种情况下,渗透都受到界面宽度和着色剂大小的影响。干燥条件下的计算表明,渗透很快,主要由毛细压力驱动,随着界面宽度的减小,渗透增加。牙本质小管及其连通程度会改变渗透深度。对于湿润条件,示踪剂大小是控制渗透长度和速度的主要因素(示踪剂越大,渗透越慢)。
我们的模型计算表明,示踪剂渗透研究必须在严格的实验条件下进行。干燥和湿润界面是两种具有非常不同示踪剂渗透模式的极端情况。体外着色剂渗透试验应在这两种情况下进行,避免缝隙中同时存在空气和水的情况。这些模型也可以适应其他牙科情况。