CSIRO Molecular and Health Technologies, Bag 10, Clayton South MDC, VIC 3169, Australia.
Int J Pharm. 2010 Aug 16;395(1-2):290-7. doi: 10.1016/j.ijpharm.2010.05.029. Epub 2010 May 24.
The preparation, characterisation and assessment of drug delivery vehicles is typically a slow and complex process. Here we present a nanostructured nanoparticle system that can be prepared and characterised in a high-throughput fashion. In particular we use phytantriol and Myverol to prepare inverse bicontinuous cubic and inverse hexagonal liquid crystalline nanoparticles loaded with 10 commonly used therapeutic agents at increasing concentration. The dispersions are prepared using automated apparatus to create different concentrations and phases using novel protocols. We are able to characterise each stabilised nanoparticle dispersion using a range of methodologies including small angle X-ray scattering, particle sizing and drug partitioning. With this information we are able to assess which drug delivery vehicle is preferred for each drug and at which concentration the drug should be loaded to ensure maximum payload and to retain particle integrity.
药物输送载体的制备、表征和评估通常是一个缓慢而复杂的过程。在这里，我们提出了一种纳米结构的纳米粒子系统，可以以高通量的方式进行制备和表征。特别是，我们使用植烷三醇和 Myverol 来制备负载有 10 种常用治疗剂的反向双连续立方和反向六方液晶纳米粒子，并逐渐增加浓度。使用自动化设备根据新方案制备分散体以形成不同的浓度和相。我们可以使用一系列方法对每种稳定的纳米粒子分散体进行表征，包括小角 X 射线散射、颗粒尺寸和药物分配。有了这些信息，我们就能够评估哪种药物输送载体最适合每种药物，以及应该在哪个浓度下加载药物，以确保最大载药量并保持颗粒完整性。
