Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, PR China.
Biotechnol Adv. 2010 Sep-Oct;28(5):628-34. doi: 10.1016/j.biotechadv.2010.05.012. Epub 2010 May 24.
Fatty acid alkyl esters, also called biodiesel, are environmentally friendly and show great potential as an alternative liquid fuel. Biodiesel is produced by transesterification of oils or fats with chemical catalysts or lipase. Immobilized lipase as the biocatalyst draws high attention because that process is "greener". This article reviews the current status of biodiesel production with immobilized lipase, including various lipases, immobilization methods, various feedstocks, lipase inactivation caused by short chain alcohols and large scale industrialization. Adsorption is still the most widely employed method for lipase immobilization. There are two kinds of lipase used most frequently especially for large scale industrialization. One is Candida antartica lipase immobilized on acrylic resin, and the other is Candida sp. 99-125 lipase immobilized on inexpensive textile membranes. However, to further reduce the cost of biodiesel production, new immobilization techniques with higher activity and stability still need to be explored.
脂肪酸烷基酯，也称为生物柴油，具有环保性，作为替代液体燃料具有巨大的潜力。生物柴油是通过化学催化剂或脂肪酶将油或脂肪进行酯交换反应而生产的。固定化脂肪酶作为生物催化剂引起了高度关注，因为该过程更加“环保”。本文综述了固定化脂肪酶生产生物柴油的现状，包括各种脂肪酶、固定化方法、各种原料、短链醇引起的脂肪酶失活以及大规模工业化。吸附仍然是最广泛使用的脂肪酶固定化方法。有两种脂肪酶最常用于大规模工业化，一种是固定在丙烯树脂上的南极假丝酵母脂肪酶，另一种是固定在廉价纺织膜上的 Candida sp. 99-125 脂肪酶。然而，为了进一步降低生物柴油生产成本，仍需要探索具有更高活性和稳定性的新型固定化技术。
