Bavarian State Collection of Zoology (ZSM), Department of Ichthyology, Münchhausenstr. 21, 81247 Munich, Germany.
Mol Phylogenet Evol. 2010 Sep;56(3):1011-24. doi: 10.1016/j.ympev.2010.05.015. Epub 2010 May 24.
Nicaraguan Midas cichlids from crater lakes have recently attracted attention as potential model systems for speciation research, but no attempt has been made to comprehensively reconstruct phylogenetic relationships of this highly diverse and recently evolved species complex. We present a first AFLP (2793 loci) and mtDNA based phylogenetic hypothesis including all described and several undescribed species from six crater lakes (Apoyeque, Apoyo, Asososca Leon, Masaya, Tiscapa and Xiloá), the two great Lakes Managua and Nicaragua and the San Juan River. Our analyses demonstrate that the relationships between the Midas cichlid members are complex, and that phylogenetic information from different markers and methods do not always yield congruent results. Nevertheless, monophyly support for crater lake assemblages from Lakes Apoyeque, Apoyo, A. Leon is high as compared to those from L. Xiloá indicating occurrence of sympatric speciation. Further, we demonstrate that a 'three species' concept for the Midas cichlid complex is inapplicable and consequently that an individualized and voucher based approach in speciation research of the Midas cichlid complex is necessary at least as long as there is no comprehensive revision of the species complex available.
尼加拉瓜裂谷湖中的米亚斯鱼最近引起了人们对物种形成研究的关注，但是，尚未有人尝试全面重建这个高度多样化且最近进化的物种复合体的系统发育关系。我们提出了第一个 AFLP（2793 个基因座）和 mtDNA 基于系统发育的假说，其中包括来自六个火山口湖（阿波耶奎、阿波约、阿索索斯卡莱昂、马萨亚、提斯卡帕和希洛阿）、两个大湖马那瓜湖和尼加拉瓜湖以及圣胡安河的所有描述和几个未描述的物种。我们的分析表明，米亚斯鱼成员之间的关系非常复杂，不同标记和方法的系统发育信息并不总是产生一致的结果。尽管如此，与来自希洛阿湖的米亚斯鱼相比，来自阿波耶奎、阿波约和阿索索斯卡莱昂湖的火山口湖组合的单系支持度较高，表明存在同域物种形成。此外，我们证明米亚斯鱼复合体的“三物种”概念不适用，因此，在没有对物种复合体进行全面修订的情况下，至少在米亚斯鱼复合体的物种形成研究中，需要采用个体化和凭证的方法。
