Tavmergen E, Tavmergen E N, Capanoglu R
Klinik für Gynäkologie und Geburtshilfe, Ege-Universität Izmir/Türkei.
Zentralbl Gynakol. 1991;113(6):317-22.
Experimental studies on animal models are necessary for the maintenance of practice and experience on IVF and ET, before starting an IVF program. Only so the handling skill of gametes zygote and human embryo culture can be improved. We have started for this reason with IVF on mouse oocytes of CB6F1 Han hybrid strains and used modified Ham's F-10 with 0.75 BSA as culture medium. Until December 1988, 3652 oocytes were collected by the superovulation of 178 oocyte donor mice. The fertilization rate was 67.94%. After the transfer of 62 hybrid embryos obtained from 4 other oocyte donor mice to 4 Balb/c recipient females, 1 recipient mouse delivered 10 living hybrid mice.
在开展体外受精（IVF）项目之前，对动物模型进行实验研究对于维持IVF和胚胎移植（ET）的实践及经验是必要的。只有这样，才能提高对配子、受精卵和人类胚胎培养的操作技能。出于这个原因，我们开始对CB6F1 Han杂交品系的小鼠卵母细胞进行IVF，并使用添加了0.75%牛血清白蛋白（BSA）的改良哈姆氏F-10培养基。到1988年12月，通过对178只卵母细胞供体小鼠进行超排卵，共采集到3652枚卵母细胞。受精率为67.94%。将从另外4只卵母细胞供体小鼠获得的62枚杂交胚胎移植到4只Balb/c受体雌性小鼠体内后，1只受体小鼠产下了10只存活的杂交小鼠。
