Leroy F, Puissant F, Camus M, Englert Y
In vitro fertilization clinic, Saint-Pierre Hospital, Free University Brussels, Belgium.
Ann Biol Clin (Paris). 1987;45(3):361-7.
The successive stages leading to fertilization in mammals are reviewed in this article. Methods of human sperm preparation for IVF are described and the "ideal" delay between oocyte pick-up and insemination time is discussed, as well as methods to reduce the incidence of polyspermy. Different culture media and their supplementation are mentioned, as well as a semi-quantitative embryonic scoring system, defined by the IVF team of the Saint-Pierre Hospital in Brussels. Finally the optimal transfer time, and the handling of embryos at replacement are discussed.
