School of Life Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, PR China.
Bioresour Technol. 2010 Nov;101(21):8383-8. doi: 10.1016/j.biortech.2010.06.052. Epub 2010 Jul 2.
A bioactivity-guided approach was employed to isolate and determine the chemical identity of bioactive constituents with antifungal activity from Actinoplanes sp. HBDN08. The structure of the antifungal metabolite was elucidated as 5-hydroxyl-5-methyl-2-hexenoic acid on the basis of spectral analysis. This compound showed strong in vitro antifungal activity against Botrytis cinerea, Cladosporium cucumerinum and Corynespora cassiicola, with an IC(50) of 32.45, 27.17, and 30.66 mg/L, respectively; however, it only moderately inhibited hyphal growth of Rhizoctonia solani with an IC(50) of 61.64 mg/L. The in vivo antifungal activity under greenhouse conditions demonstrated that 5-hydroxyl-5-methyl-2-hexenoic acid could effectively control the diseases caused by B. cinerea, C. cucumerinum and C. cassiicola with 71.42%, 78.63% and 65.13% control values at 350 mg/L, respectively. This strong antifungal activity suggests that 5-hydroxyl-5-methyl-2-hexenoic acid might be a promising candidate for new antifungal agents.
采用生物活性导向方法，从链霉菌属 HBDN08 中分离并鉴定具有抗真菌活性的生物活性成分。根据光谱分析，确定该抗真菌代谢产物的结构为 5-羟基-5-甲基-2-己烯酸。该化合物对灰葡萄孢、黄瓜炭疽菌和姜炭疽菌表现出较强的体外抗真菌活性，IC50 值分别为 32.45、27.17 和 30.66mg/L；然而，它对茄丝核菌的菌丝生长仅表现出中等抑制作用，IC50 值为 61.64mg/L。温室条件下的体内抗真菌活性表明，5-羟基-5-甲基-2-己烯酸在 350mg/L 时可有效控制灰葡萄孢、黄瓜炭疽菌和姜炭疽菌引起的病害，防治效果分别为 71.42%、78.63%和 65.13%。这种强烈的抗真菌活性表明，5-羟基-5-甲基-2-己烯酸可能是一种有前途的新型抗真菌药物候选物。
