在光生物反应器中高浓度 CO2 下小球藻 765 的生长特性。
Growth characteristics of Botryococcus braunii 765 under high CO2 concentration in photobioreactor.
机构信息
Department of Environmental Engineering, Zhejiang University, Hangzhou 310029, China.
出版信息
Bioresour Technol. 2011 Jan;102(1):130-4. doi: 10.1016/j.biortech.2010.06.051. Epub 2010 Jun 26.
To understand the potential of cultivating Botryococcus braunii with flue gas (normally containing high CO(2)) for biofuel production, growth characteristics of B. braunii 765 with 2-20% CO(2) aeration were investigated. The results showed that the strain could grow well without any obvious inhibition under all tested CO(2) concentrations with an aeration rate of 0.2 vvm, even without any culture pH adjustment (ranged from 6.0 to 8.0). The maximum biomass among all conditions was 2.31 g L(-1) on 25th day at 20% CO(2). Hydrocarbon content and algal colony size increased with the increase of CO(2) concentration. A negative correlation between algal biomass and culture total phosphorus was observed (from -0.828 to -0.911, P<0.01). Additionally, 2% sodium hypochlorite solution was used for photobioreactor sterilization to cultivate B. braunii.
为了了解利用富含二氧化碳的烟道气（通常含有高浓度的 CO₂）培养 Botryococcus braunii 以生产生物燃料的潜力，研究了 B. braunii 765 在 2-20% CO₂通气条件下的生长特性。结果表明，在 0.2 vvm 的通气速率下，该菌株在所有测试的 CO₂浓度下均能良好生长，没有明显的抑制作用，甚至无需进行任何培养 pH 调节（范围为 6.0 至 8.0）。在 20% CO₂条件下，第 25 天时最大生物量达到 2.31 g/L。烃含量和藻类菌落大小随 CO₂浓度的增加而增加。观察到藻类生物量与培养液总磷之间呈负相关（从-0.828 到-0.911，P<0.01）。此外，还使用 2%次氯酸钠溶液对光生物反应器进行消毒，以培养 B. braunii。
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