在户外条件下，于跑道式和圆形池塘中培养绿藻 Botryococcus braunii 及其生长和烃类生产。
Cultivation of green alga Botryococcus braunii in raceway, circular ponds under outdoor conditions and its growth, hydrocarbon production.
机构信息
Plant Cell Biotechnology Department, CSIR-Central Food Technological Research Institute, Mysore 570 020, India.
出版信息
Bioresour Technol. 2012 Nov;123:528-33. doi: 10.1016/j.biortech.2012.07.009. Epub 2012 Jul 26.
The present study focused on cultivation, seasonal variation in growth, hydrocarbon production, fatty acids profiles of Botryococcus braunii (LB-572 and N-836) in raceway & circular ponds under outdoor conditions. After 18days of cultivation the biomass yield and hydrocarbon contents were increased in both raceway and circular ponds. The fat content was found to be around 24% (w/w) with palmitic and oleic acids as prominent fatty acids. Hydrocarbons of C(20)-C(30) carbon chain length were higher in raceway and circular ponds. Maximum biomass yield (2gL(-1)) and hydrocarbon content (28%) were observed in Nov-Dec. In case of B. braunii (N-836) after 25days of cultivation the biomass yield was 1gL(-1) and hydrocarbon content was 27%. Supplementation of 0.1% NaHCO(3) in the medium resulted in biomass yield of 1.5gL(-1) and hydrocarbon content of 30% compared to control.
本研究聚焦于在户外条件下，在跑道和圆形池塘中培养、生长的季节性变化、产烃、Botryococcus braunii（LB-572 和 N-836）的脂肪酸谱。培养 18 天后，跑道和圆形池塘中的生物量产量和烃含量都有所增加。脂肪含量约为 24%（w/w），棕榈酸和油酸是主要的脂肪酸。C（20）-C（30）碳链长度的碳氢化合物在跑道和圆形池塘中含量较高。11 月至 12 月观察到最大生物量产量（2gL(-1)）和烃含量（28%）。对于 B. braunii（N-836），培养 25 天后，生物量产量为 1gL(-1)，烃含量为 27%。与对照相比，在培养基中添加 0.1%的 NaHCO(3)可使生物量产量达到 1.5gL(-1)，烃含量达到 30%。
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