Gonzalez R, Griparic L, Umana M, Burgee K, Vargas V, Nasrallah R, Silva F, Patel A
DaVinci Biosciences LLC, Costa Mesa, CA 92627, USA.
Cell Transplant. 2010;19(11):1439-49. doi: 10.3727/096368910X514260. Epub 2010 Jun 29.
There have been various forms of mesenchymal stem cell-like (MSC-like) cells isolated from umbilical cords (UCs). The isolation of umbilical cord lining stem cells (ULSCs) may be of great value for those interested in a possible treatment to several disease/disorders. Unlike umbilical cord blood cells, these cells are unique because they can be expanded to therapeutically relevant numbers and cryopreserved for several different uses. Here we efficiently isolate stem cells from a small segment of pre- and postnatal UCs, and obtain therapeutically relevant amounts of ULSCs within 3 weeks. We demonstrate their growth potential and characterize them using immunocytochemistry, flow cytometry, and RT-PCR. In addition, we differentiate ULSCs into multiple lineages. Pre- and postnatal ULSCs are morphologically similar to mesenchymal stem cells (MSCs) and easily expand to greater than 70 population doublings. They express pluripotent markers Oct4 and nanog at the protein and RNA level. Flow cytometry demonstrates that they express markers indicative of MSCs in addition to high SSEA-4 expression. ULSCs are easily differentiated into osteogenic, adipogenic, chondrogenic, cardiogenic, and neurogenic cells. Pre- and postnatal ULSCs are characteristically similar in respect to their growth, marker expression, and plasticity, demonstrating they are highly conserved throughout development. ULSCs have phenotypic and genotypic properties of MSCs. These studies demonstrate the therapeutic potential of an otherwise discarded tissue. They are a perfect HLA match for the donor and an excellent match for immediate family members; therefore, they may serve as a therapeutic cell source.
已经从脐带（UC）中分离出了多种间充质干细胞样（MSC样）细胞。对于那些对几种疾病/病症的可能治疗方法感兴趣的人来说，脐带内衬干细胞（ULSC）的分离可能具有很大的价值。与脐带血细胞不同，这些细胞是独特的，因为它们可以扩增到治疗相关数量，并可冷冻保存用于多种不同用途。在这里，我们有效地从一小段产前和产后脐带中分离出干细胞，并在3周内获得治疗相关数量的ULSC。我们展示了它们的生长潜力，并使用免疫细胞化学、流式细胞术和逆转录-聚合酶链反应对其进行了表征。此外，我们将ULSC分化为多个谱系。产前和产后的ULSC在形态上与间充质干细胞（MSC）相似，并且很容易扩增到超过70个群体倍增。它们在蛋白质和RNA水平上表达多能性标志物Oct4和nanog。流式细胞术表明，除了高表达阶段特异性胚胎抗原-4（SSEA-4）外，它们还表达指示MSC的标志物。ULSC很容易分化为成骨细胞、脂肪细胞、软骨细胞、心肌细胞和神经细胞。产前和产后的ULSC在生长、标志物表达和可塑性方面具有特征性相似性，表明它们在整个发育过程中高度保守。ULSC具有MSC的表型和基因型特性。这些研究证明了一种原本被丢弃的组织的治疗潜力。它们与供体的人类白细胞抗原（HLA）完全匹配，与直系亲属也非常匹配；因此，它们可以作为一种治疗细胞来源。
