Cumaoglu Ahmet, Stefek Milan, Bauer Viktor, Ari Nuray, Aricioglu Aysel, Karasu Cimen
Department of Medical Pharmacology, Faculty of Medicine Gazi University, Ankara, Turkey.
Neuro Endocrinol Lett. 2010;31(3):313-8.
Recent basic research and clinical data have provided new insights into the role of glycoxidative and nitrosative stresses (both oxidative stress) in diabetic complications, such as diabetic nephropathy, suggesting a different and innovative approach to antioxidant therapy. In streptozotocin-induced diabetic rat kidney, the present study investigated the effects of the synthetic pyridoindole antioxidant stobadine (STB) on renal total antioxidant potential (AOP) and protein oxidation parameters such as protein carbonyl content (PCC), advanced oxidation protein products (AOPPs) and nitrotyrosine (NT), a marker specific for protein modification by peroxynitrite.
Wistar Albino rats were divided into two groups: normal and streptozotocin-induced diabetic rats. Each group of the animals was further divided into two groups: untreated and treated with stobadine (24.7 mg/kg) during 16 weeks daily by oral gavage.
The renal tissue AOP and the levels of AOPPs, PCC and NT were increased in diabetic rats compared with the untreated control animals. Furthermore, stobadine treatment significantly decreased protein carbonylation and AOPPs but not NT.
These findings indicate that STB is an antioxidant factor which can improve glycoxidative stress markers in kidney, while it has no effect on protein nitrosylation.
近期的基础研究和临床数据为糖氧化应激和亚硝化应激（均为氧化应激）在糖尿病并发症（如糖尿病肾病）中的作用提供了新的见解，提示了一种不同的、创新的抗氧化治疗方法。在链脲佐菌素诱导的糖尿病大鼠肾脏中，本研究调查了合成的吡啶吲哚抗氧化剂司他丁（STB）对肾脏总抗氧化能力（AOP）以及蛋白质氧化参数（如蛋白质羰基含量（PCC）、晚期氧化蛋白产物（AOPPs）和硝基酪氨酸（NT），一种过氧亚硝酸盐对蛋白质修饰的特异性标志物）的影响。
将Wistar白化大鼠分为两组：正常大鼠和链脲佐菌素诱导的糖尿病大鼠。每组动物进一步分为两组：未治疗组和通过每日口服灌胃给予司他丁（24.7 mg/kg）治疗16周的治疗组。
与未治疗的对照动物相比，糖尿病大鼠的肾脏组织AOP以及AOPPs、PCC和NT水平升高。此外，司他丁治疗显著降低了蛋白质羰基化和AOPPs，但对NT没有影响。
这些发现表明，STB是一种抗氧化因子，可改善肾脏中的糖氧化应激标志物，而对蛋白质亚硝化没有影响。
