Ashok P C, Singh G P, Tan K M, Dholakia K
SUPA, School of Physics and Astronomy, University of St Andrews, North Haugh, Fife, KY16 9SS, UK.
Opt Express. 2010 Apr 12;18(8):7642-9. doi: 10.1364/OE.18.007642.
We report a novel fiber probe based Raman detection system on a microfluidic platform where a split Raman probe is directly embedded into a polydimethylsiloxane (PDMS) chip. In contrast to previous Raman detection schemes in microfluidics, probe based detection offers reduced background and portability. Compared to conventional backscattering probe designs, the split fiber probe we used in this system, results in a reduced size and offers flexibility to modify the collection geometry to minimize the background generated by the fibers. Also our microfluidic chip design enables us to obtain an alignment free system. As a proof of concept we demonstrate the sensitivity of the device for urea detection at relevant human physiological levels with a low acquisition time. The development of this system on a microfluidic platform means portable, lab on a chip devices for biological analyte detection and environmental sensing using Raman spectroscopy are now within reach.
我们报道了一种基于微流控平台的新型光纤探针拉曼检测系统，其中一个分离式拉曼探针直接嵌入聚二甲基硅氧烷（PDMS）芯片中。与微流控中先前的拉曼检测方案相比，基于探针的检测具有更低的背景和更高的便携性。与传统的背散射探针设计相比，我们在该系统中使用的分离式光纤探针尺寸更小，并且能够灵活修改采集几何结构，以尽量减少光纤产生的背景。此外，我们的微流控芯片设计使我们能够获得一个无需对准的系统。作为概念验证，我们展示了该设备在相关人体生理水平下检测尿素的灵敏度，且采集时间较短。在微流控平台上开发此系统意味着，现在可以实现用于生物分析物检测和环境传感的便携式芯片实验室设备，这些设备使用拉曼光谱技术。
