Neural Circuit Development Laboratory, Institut de recherches cliniques de Montréal (IRCM), 110 avenue des Pins Ouest, Montréal , QC, Canada, H2W 1R7.
Dev Dyn. 2010 Aug;239(8):2246-55. doi: 10.1002/dvdy.22357.
During limb development, the dorsal limb mesenchyme expression of the transcription factor LMX1B is required for dorsoventral limb patterning. In mice, Lmx1b mutations result in the mirror-image duplication of ventral limb structures and loss of dorsal limb structures. Heterozygous LMX1B mutations in humans cause the Nail-Patella Syndrome characterized by limb, kidney, and eye developmental defects. We used DNA microarrays to compare the mRNAs in E13.5 mouse Lmx1b mutant and wild-type limbs. We report 14 genes that require Lmx1b for their normal expression in the dorsal limb or the restriction of their expression to the ventral limb.
在肢体发育过程中，转录因子 LMX1B 的背侧肢体间充质表达对于肢体的背腹模式形成是必需的。在小鼠中，Lmx1b 突变导致腹侧肢体结构的镜像重复和背侧肢体结构的缺失。人类杂合 LMX1B 突变导致指甲-髌骨综合征，其特征为肢体、肾脏和眼睛发育缺陷。我们使用 DNA 微阵列比较了 E13.5 鼠标 Lmx1b 突变体和野生型肢体中的 mRNA。我们报告了 14 个基因，它们的正常表达需要 Lmx1b 来在背侧肢体中进行，或者将其表达限制在腹侧肢体中。
