对艾伯塔省出现临床猪圆环病毒相关疾病（PCVAD）的猪群中2型猪圆环病毒分离株进行基因组分析。
Genomic analysis of porcine circovirus type-2 isolates in Alberta pigs demonstrating clinical porcine circovirus associated disease (PCVAD).
作者信息
McIntyre Leila, Chaiyakul Mark, Clark Edward G, Marshall Frank, Czub Markus
机构信息
Department of Production Animal Health, University of Calgary, Alberta, Canada.
出版信息
Can J Vet Res. 2010 Apr;74(2):149-52.
Nineteen pigs with clinical signs of porcine circovirus associated diseases (PCVAD) on 5 Alberta pig farms were examined pathologically, including gross pathology, histopathology, and immunohistochemistry. Polymerase chain reaction (PCR) for porcine circovirus type-2 (PCV-2) and sequence analysis was performed on tissue samples of 12 animals. Results showed that new strains of porcine circovirus type-2 genogroup b were present in most pigs that were positive for PCV-2. Furthermore, a mixed infection with PCV-2a and PCV-2b occurred in the liver and lungs of 1 pig. Comparison of whole genome sequences representing known viruses and the newly discovered Alberta viruses revealed mutations distributed over the entire genome of PCV-2; however, sequences encoding for immunodominant epitopes of PCV-2 appear to be unaffected by these mutations.
对艾伯塔省5个养猪场出现猪圆环病毒相关疾病（PCVAD）临床症状的19头猪进行了病理检查，包括大体病理学、组织病理学和免疫组织化学检查。对12头猪的组织样本进行了2型猪圆环病毒（PCV-2）的聚合酶链反应（PCR）和序列分析。结果显示，在大多数PCV-2呈阳性的猪中存在2型猪圆环病毒b基因型的新毒株。此外，1头猪的肝脏和肺脏发生了PCV-2a和PCV-2b的混合感染。对代表已知病毒的全基因组序列与新发现的艾伯塔病毒进行比较，发现PCV-2的整个基因组存在突变；然而，编码PCV-2免疫显性表位的序列似乎未受这些突变影响。
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