NCMH, University of Nottingham, School of Biosciences, Sutton Bonington, Leicestershire, UK.
Macromol Biosci. 2010 Jul 7;10(7):798-807. doi: 10.1002/mabi.201000065.
Fitting r = f(c) as opposed to the usual c = f(r) to the inverted form of the sedimentation equilibrium equation for interacting solute (INVEQ algorithm), it is shown by detailed simulation and by experimentation that stable, simultaneous estimates can be retrieved for both virial (2nd BM/3rd CM) and specific interaction (K(a)) terms. In suitable systems estimates for two distinct second virial (BM) and single K(a) terms can equally be defined. Whilst cell loading level is critical, noise level in the interference fringe data is shown to have surprisingly little influence on these outcomes.
将 r = f(c) 而不是通常的 c = f(r) 拟合到相互作用溶质的沉降平衡方程的倒易形式（INVEQ 算法），通过详细的模拟和实验表明，可以同时稳定地检索到双分子（2 阶 BM/3 阶 CM）和特异性相互作用（K(a)）项的估算值。在合适的系统中，也可以定义两个不同的第二维里（BM）和单个 K(a)项的估计值。虽然细胞加载水平很关键，但干扰条纹数据中的噪声水平对这些结果的影响出人意料地小。
