School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, Korea.
Electrophoresis. 2010 Jul;31(13):2137-53. doi: 10.1002/elps.200900682.
Rapid and sensitive detection of food-borne pathogens is critical for food safety from the viewpoint of both the public health professionals and the food industry. Conventional method is, however, known to be labor-intensive, time-consuming, and expensive due to the separate cultivation and biochemical assay. Many relevant technologies, such as flow cytometry, MALDI-MS, ESI-MS, DNA microarray, and CE, have been intensively developed to date. Among them, CE is considered to be the most efficient and reproducible because of low sample loss and simple automation. CE-based pathogen detection methods can be classified into three categories based on the separation targets: cell separation, nucleic-acid-based identification, and protein separation coupled with characterization. In this review, recent developments in each sphere of CE-based technology are discussed. Additionally, the critical features of each approach and necessary future technical improvements are also reviewed.
从公共卫生专业人员和食品行业的角度来看，快速、灵敏地检测食源性病原体至关重要。然而，由于需要单独培养和生化分析，传统方法已知是劳动密集型、耗时且昂贵的。迄今为止，已经开发了许多相关技术，例如流式细胞术、MALDI-MS、ESI-MS、DNA 微阵列和 CE。其中，CE 由于样品损失低和自动化简单，被认为是最有效和可重复的。基于 CE 的病原体检测方法可以根据分离目标分为三类：细胞分离、基于核酸的鉴定以及与特性相结合的蛋白质分离。在本综述中，讨论了基于 CE 的技术在各个领域的最新进展。此外，还审查了每种方法的关键特征和必要的未来技术改进。
