Minnesota Department of Health, USA.
Curr Opin Biotechnol. 2010 Apr;21(2):137-41. doi: 10.1016/j.copbio.2010.02.011. Epub 2010 Mar 17.
Current subtyping methods for the detection of foodborne disease outbreaks have limitations that reduce their use by public health laboratories. Recent advances in subtyping of foodborne disease pathogens utilize techniques that identify nucleic acid polymorphisms. Recent methods of nucleic acid characterization such as microarrays and mass spectrometry (MS) may provide improvements such as increasing speed and data portability while decreasing labor compared to current methods. This article discusses multiple-locus variable-number tandem-repeat analysis, single-nucleotide polymorphisms, nucleic acid sequencing, whole genome sequencing, variable absent or present loci, microarrays and MS as potential subtyping methods to enhance our ability to detect foodborne disease outbreaks.
当前用于检测食源性疾病暴发的亚型方法存在局限性，这限制了公共卫生实验室对其的使用。食源性疾病病原体亚型分析的最新进展利用了识别核酸多态性的技术。与当前方法相比，核酸特征的最新方法，如微阵列和质谱 (MS)，可能会提供改进，例如提高速度和数据可移植性，同时减少劳动力。本文讨论多位点可变数串联重复分析、单核苷酸多态性、核酸测序、全基因组测序、可变缺失或存在基因座、微阵列和 MS 作为潜在的亚型方法，以增强我们检测食源性疾病暴发的能力。
