Fotadar U, Shapiro L E, Surks M I
Department of Medicine, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, NY 10467.
Biotechniques. 1991 Feb;10(2):171-2.
In this communication we describe the sequential use of standard and low-melting agarose in a single gel slab for the electrophoresis of DNA. This method has the advantages of high resolution and reproducibility characteristic of standard agarose and the ease of manipulation of DNA for direct cloning, sequential digestion and isolation, characteristic of low-melting agarose.
在本交流中，我们描述了在单个凝胶板中顺序使用标准琼脂糖和低熔点琼脂糖进行DNA电泳的方法。该方法具有标准琼脂糖的高分辨率和可重复性优点，以及低熔点琼脂糖便于直接克隆、顺序消化和分离DNA的操作特点。
