Department of Life Science, Sogang University, Seoul, Republic of Korea.
Microb Pathog. 2010 Oct;49(4):174-80. doi: 10.1016/j.micpath.2010.05.009. Epub 2010 Jun 2.
We have previously shown that PQS and HHQ, two quorum sensing molecules, can down-regulate host the innate immune responses and that this is mediated through the NF-kappaB pathway. In this study, to search for a comprehensive set of genes regulated by these quorum sensing molecules, we performed a global gene expression analysis using DNA microarray in J774A.1 monocyte/macrophage cells line. The expression of these genes was confirmed by RT-PCR. We found that PQS and HHQ down-regulated the expression of genes involved in immune responses and transcription as well as other functions, some of which are downstream of NF-kappaB pathway consistent with our previous results. PQS and HHQ inhibited LPS-induced morphological change and nitric oxide production, suggesting that they inhibit macrophage activation. However, PQS and HHQ did not affect apoptosis, suggesting that their effects on immune system are not from general alteration of cell function. This study provides insight how the quorum sensing molecules influence host cells.
我们之前已经表明，两种群体感应分子 PQS 和 HHQ 可以下调宿主固有免疫反应，而这是通过 NF-κB 途径介导的。在这项研究中，为了寻找这些群体感应分子调控的一组全面的基因，我们使用 DNA 微阵列在 J774A.1 单核细胞/巨噬细胞系中进行了全局基因表达分析。这些基因的表达通过 RT-PCR 进行了确认。我们发现，PQS 和 HHQ 下调了参与免疫反应和转录以及其他功能的基因的表达，其中一些是 NF-κB 途径的下游基因，与我们之前的结果一致。PQS 和 HHQ 抑制 LPS 诱导的形态变化和一氧化氮的产生，表明它们抑制巨噬细胞的激活。然而，PQS 和 HHQ 并不影响细胞凋亡，这表明它们对免疫系统的影响不是来自于细胞功能的普遍改变。这项研究提供了关于群体感应分子如何影响宿主细胞的深入了解。
