Department of Electrical Engineering, California Institute of Technology, 1200 E. California Boulevard, Pasadena, California 91125, USA.
Opt Lett. 2010 Jul 1;35(13):2188-90. doi: 10.1364/OL.35.002188.
We have developed a new microscopy design that can achieve wide field-of-view (FOV) imaging and yet possesses resolution that is comparable to a conventional microscope. In our design, the sample is illuminated by a holographically projected light-spot grid. We acquire images by translating the sample across the grid and detecting the transmissions. We have built a prototype system with an FOV of 6 mm x 5 mm and acquisition time of 2.5 s. The resolution is fundamentally limited by the spot size--our demonstrated average FWHM spot diameter was 0.74 microm. We demonstrate the prototype by imaging a U.S. Air Force target and a lily anther. This technology is scalable and represents a cost-effective way to implement wide FOV microscopy systems.
我们开发了一种新的显微镜设计,可以实现大视场(FOV)成像,并且分辨率可与传统显微镜相媲美。在我们的设计中,样品被全息投影的光斑网格照明。我们通过在网格上平移样品并检测透射光来获取图像。我们已经构建了一个 FOV 为 6mm×5mm、采集时间为 2.5s 的原型系统。分辨率受到光斑尺寸的限制,我们演示的平均 FWHM 光斑直径为 0.74 微米。我们通过对美国空军靶标和百合花粉囊进行成像来演示该原型系统。这项技术具有可扩展性,代表了实现大 FOV 显微镜系统的一种具有成本效益的方法。