使用平板扫描仪的0.5千兆像素显微镜技术。
0.5 gigapixel microscopy using a flatbed scanner.
作者信息
Zheng Guoan, Ou Xiaoze, Yang Changhuei
机构信息
Electrical Engineering, California Institute of Technology, Pasadena, CA, 91125, USA ; Presently at Biomedical Engineering & Electrical Engineering, University of Connecticut, Storss, CT, 06269 USA ; These authors contributed equally to this work.
Electrical Engineering, California Institute of Technology, Pasadena, CA, 91125, USA ; These authors contributed equally to this work.
出版信息
Biomed Opt Express. 2013 Dec 2;5(1):1-8. doi: 10.1364/BOE.5.000001.
The capability to perform high-resolution, wide field-of-view (FOV) microscopy imaging is highly sought after in biomedical applications. In this paper, we report a wide FOV microscopy system that uses a closed-circuit-television (CCTV) lens for image relay and a flatbed scanner for data acquisition. We show that such an imaging system is capable of capturing a 10 mm × 7.5 mm FOV image with 0.78 µm resolution, resulting in more than 0.5 billion pixels across the entire image. The resolution and field curve of the proposed system were characterized by imaging a USAF resolution target and a hole-array target. To demonstrate its application, 0.5 gigapixel images of histology slides were acquired using this system.
在生物医学应用中，对能够进行高分辨率、宽视场（FOV）显微镜成像的能力有着极高的需求。在本文中，我们报告了一种宽视场显微镜系统，该系统使用闭路电视（CCTV）镜头进行图像中继，并使用平板扫描仪进行数据采集。我们表明，这样的成像系统能够以0.78 µm的分辨率捕获10 mm×7.5 mm视场的图像，整个图像的像素超过5亿。通过对美国空军分辨率靶标和孔阵列靶标成像，对所提出系统的分辨率和场曲线进行了表征。为了展示其应用，使用该系统采集了组织学切片的5亿像素图像。
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