Faculty of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa 32000, Israel.
Opt Lett. 2010 Jul 1;35(13):2218-20. doi: 10.1364/OL.35.002218.
Flow cytometry techniques often rely on detecting fluorescence from single cells flowing through the cross section of a laser beam, providing invaluable information on vast numbers of cells. Such techniques, however, are often limited in their ability to resolve clusters of cells or parallel cell flow through large vessels. We present a confocal imaging technique that images unstained cells flowing in parallel through a wide channel, using spectrally encoded reflectance confocal microscopy that does not require mechanical scanning. Images of red blood cells from our system are compared to conventional transmission microscopy, and imaging of flowing red blood cells in vitro is experimentally demonstrated.
流式细胞术技术通常依赖于检测穿过激光束横截面的单细胞的荧光，从而提供大量细胞的有价值的信息。然而，这些技术通常在解析细胞簇或平行流过大血管的细胞流方面的能力有限。我们提出了一种共焦成像技术，该技术使用无需机械扫描的光谱编码反射共焦显微镜对平行流过宽通道的未染色细胞进行成像。我们系统的红细胞图像与传统的透射显微镜进行了比较，并实验证明了体外流动红细胞的成像。
