在用致畸剂环己酰亚胺治疗后，一只怀孕大鼠身上出现了乳腺肿瘤。
Breast tumor developed in a pregnant rat after treatment with the teratogen Cycloheximide.
作者信息
Emmanouil-Nikoloussi E N, Nikoloussis E, Manthou M E, Goula O Ch, Likartsis Ch, Papamitsou Th, Frangou H, Massouridou S, Lazaridis Ch, Manthos A
出版信息
Hippokratia. 2010 Apr;14(2):136-8.
AIM
To describe histochemically and immunohistochemically a breast tumor presented in a pregnant rat given Cycloheximide to examine its teratogenic and embryotoxic effect on the embryos.
METHODS
Cycloheximide was injected in pregnant Wistar rats at a dose of 3 mg/kg.b.w. on both 10th and 11th gestational days. In one of the rats, a large breast tumor developed rapidly. Histochemical staining with Hematoxylin-Eosin and Masson Trichrome and immunohistochemical identification with mouse monoclonal antibodies: a) Estrogen Receptor A and b) Estrogen Receptor B was performed.
RESULTS
Analysis with A-receptor and B-receptor showed that the breast tumor which was developed after treatment with Cycloheximide was malignant. Positive immunohistochemical reaction was evident especially with A-receptor indicating the malignancy of the tumor.
CONCLUSIONS
Cycloheximide is a known toxic and teratogenic agent and potentially a carcinogenic drug. Thus it should be used with extreme caution as a pesticide.
目的
通过组织化学和免疫组织化学方法描述在给予孕鼠环己酰亚胺后出现的乳腺肿瘤，以研究其对胚胎的致畸和胚胎毒性作用。
方法
在妊娠第10天和第11天，以3mg/kg体重的剂量给妊娠的Wistar大鼠注射环己酰亚胺。在其中一只大鼠中，一个大的乳腺肿瘤迅速发展。进行苏木精-伊红和马松三色染色的组织化学染色，并用小鼠单克隆抗体进行免疫组织化学鉴定：a）雌激素受体A和b）雌激素受体B。
结果
A受体和B受体分析表明，用环己酰亚胺处理后出现的乳腺肿瘤是恶性的。免疫组织化学反应阳性尤其在A受体方面明显，表明肿瘤具有恶性。
结论
环己酰亚胺是一种已知的有毒和致畸剂，可能还是一种致癌药物。因此，作为杀虫剂使用时应极其谨慎。
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