McLaughlin Pamela D, McLaughlin Wayne A, Maxwell Douglas P, Roye Marcia E
Department of Basic Medical Sciences, Biochemistry Section, University of the West Indies, Mona, Kingston 7, Jamaica.
Plant Viruses. 2008;2(1):58-63.
Plants including pepper, red kidney bean, squash, string bean and tomato, as well as weeds with viral symptoms were collected from five districts in Belize over a three year period with the aim of determining the diversity of the begomoviruses present. Sixty five percent of the samples screened via DNA hybridization produced signals indicative of begomovirus infection. Subsequent PCR amplifications and nucleotide sequence analyses revealed the presence of four begomoviruses in Belize. Pepper golden mosaic virus and Tomato mottle virus-[Flo] were found associated with tomato and sweet pepper and the former was also isolated from hot pepper. Merremia mosaic virus was found infecting hot pepper, sweet pepper and the weed species Euphorbia heterophylla. Euphorbia mosaic virus-[Yucatan Peninsula] was found in hot pepper and Euphorbia. This is the first report of the identification of begomoviruses in Belize.
在三年时间里，从伯利兹的五个地区采集了包括辣椒、红芸豆、南瓜、菜豆和番茄在内的植物以及出现病毒症状的杂草，目的是确定当地存在的双生病毒的多样性。通过DNA杂交筛选的样本中，65%产生了表明双生病毒感染的信号。随后的PCR扩增和核苷酸序列分析显示，伯利兹存在四种双生病毒。辣椒金色花叶病毒和番茄斑驳病毒-[弗洛]与番茄和甜椒有关，前者也从辣椒中分离出来。发现茑萝花叶病毒感染辣椒、甜椒和杂草异叶大戟。在辣椒和大戟中发现了大戟花叶病毒-[尤卡坦半岛]。这是伯利兹双生病毒鉴定的首次报告。
