Phytopathology. 1998 Dec;88(12):1262-8. doi: 10.1094/PHYTO.1998.88.12.1262.
ABSTRACT Seven crop and eight weed species from 12 agricultural locations in Trinidad and Tobago were assayed for the presence of whitefly-transmitted geminiviruses (WTGs) by using dot blot hybridization and polymerase chain reaction (PCR) amplification of the N-terminal coat protein sequence with degenerate primers. The amplified fragments were cloned and analyzed by restriction enzyme digestion to determine fragment length polymorphism among the cloned fragments. Representative clones were then sequenced and subjected to phylogenetic analysis to determine the sequence similarity to known WTGs. WTGs were found in every location sampled and in 10 of the 15 species investigated: Lycopersicon esculentum(tomato), Capsicum annuum (pepper), Capsicum frutescens (sweet pepper), Abelmoschus esculentus (okra), Phaseolus vulgaris (beans), Alternanthera tenella, Desmodium frutescens, Euphorbia heterophylla, Malva alceifolia, and Sida acuta. The geminiviruses infecting these plants were closely related to potato yellow mosaic virus from Venezuela (PYMV-VE) and tomato leaf curl virus from Panama (ToLCV-PA). However, in pepper, sweet pepper, okra, Alternanthera tenella, Euphorbia heterophylla, Des-modium frutescens, and in one sample of tomato, a PYMV-VE-related virus was found in mixed infections with a virus related to pepper huasteco virus. Full-length infectious DNA-A and DNA-B of a tomato-infecting geminivirus from Trinidad and Tobago were cloned and sequenced. DNA-A appears to be a recombinant derived from PYMV-VE or ToLCV-PA, and Sida golden mosaic from Honduras. The implications of these findings in the control of WTGs are discussed.
摘要 本研究采用斑点杂交和聚合酶链反应（PCR）扩增，使用简并引物扩增 N 端外壳蛋白序列，对特立尼达和多巴哥 12 个农业地点的 7 种作物和 8 种杂草中的 12 种农业地点的 7 种作物和 8 种杂草中的 7 种作物和 8 种杂草进行了检测，以检测是否存在粉虱传播的双生病毒（WTG）。扩增片段用限制性内切酶消化进行克隆和分析，以确定克隆片段之间的片段长度多态性。然后对代表克隆进行测序，并进行系统发育分析，以确定与已知 WTG 的序列相似性。在每个采样地点都发现了 WTG，并在 15 种调查物种中的 10 种中发现了 WTG：番茄、辣椒、甜椒、黄秋葵、菜豆、空心菜、野茼蒿、含羞草、大戟和钝叶决明。感染这些植物的双生病毒与来自委内瑞拉的马铃薯黄花叶病毒（PYMV-VE）和来自巴拿马的番茄曲叶病毒（ToLCV-PA）密切相关。然而，在辣椒、甜椒、黄秋葵、空心菜、大戟、含羞草、野茼蒿和番茄的一个样本中，与 PYMV-VE 相关的病毒与与胡椒 Huasteco 病毒相关的病毒混合感染。从特立尼达和多巴哥分离并测序了一种感染番茄的双生病毒的全长传染性 DNA-A 和 DNA-B。DNA-A 似乎是源自 PYMV-VE 或 ToLCV-PA 和来自洪都拉斯的 Sida 金黄镶嵌病毒的重组体。讨论了这些发现对 WTG 控制的影响。
