Teodor Eugenia, Liţescu Simona Carmen, Petcu C, Mihalache Mihaela, Somoghi Raluca
Nanoscale Res Lett. 2009 Mar 6;4(6):544-549. doi: 10.1007/s11671-009-9278-x.
Magnetic nanoparticles were obtained using an adjusted Massart method and were covered in a layer-by-layer technique with hydrogel-type biocompatible shells, from chitosan and hyaluronic acid. The synthesized nanocomposites were characterized using dynamic light scattering, transmission electron microscopy, and Fourier transformed infrared spectroscopy. Biocompatibility of magnetic nanostructures was determined by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) cell proliferation assay, swelling tests, and degradation tests. In addition, interaction of hydrogel-magnetic nanoparticles with microorganisms was studied. The possibility of precise nanoparticles size control, as long as the availability of bio-compatible covering, makes them suitable for biomedical applications.
采用改良的马萨特方法制备磁性纳米颗粒，并通过层层技术用壳聚糖和透明质酸制成的水凝胶型生物相容性外壳进行包覆。使用动态光散射、透射电子显微镜和傅里叶变换红外光谱对合成的纳米复合材料进行表征。通过MTT（3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四氮唑溴盐）细胞增殖试验、溶胀试验和降解试验来确定磁性纳米结构的生物相容性。此外，还研究了水凝胶磁性纳米颗粒与微生物的相互作用。只要具备生物相容性覆盖层，精确控制纳米颗粒尺寸的可能性使其适用于生物医学应用。
