Schulz-Key H
Tropenmed Parasitol. 1978 Mar;29(1):51-4.
A technique for the assessment of the total number of microfilariae in skin snips from onchocerciasis patients is described. For the digestion of the skin tissue the biopsies are incubated at room temperature for 24 hours in medium 199, containing 3 mg/ml collagenase and antibiotics. This process leaves the microfilariae unharmed. A comparative study of the emergence of the microfilariae in different media showed that only 20% of the total number were released after 30 minutes in distilled water. After 24 hours incubation in isotonic saline 80% were found.
本文描述了一种评估盘尾丝虫病患者皮肤切片中微丝蚴总数的技术。为消化皮肤组织，活检样本在含有3mg/ml胶原酶和抗生素的199培养基中于室温下孵育24小时。此过程不会伤害微丝蚴。对微丝蚴在不同培养基中逸出情况的比较研究表明，在蒸馏水中30分钟后仅释放出总数的20%。在等渗盐水中孵育24小时后，发现释放出80%。
