Awadzi K, Roulet H, Bell D R
Ann Trop Med Parasitol. 1980 Jun;74(3):363-6.
The microfilarial density in 400 skin snips from 100 patients was determined using the standard technique of the Onchocerciasis Chemotherapeutic Research Centre (OCRC method), the method used by the Onchocerciasis Control Programme (OCP method), and also after collagenase digestion. The OCRC method was fairly consistent and detected 84% of the total microfilariae. The OCP method gave a density which was consistently 20% less than the OCRC method, indicating that the increase in weight in skin snips following incubation in saline was fairly predictable. It is concluded that collagenase digestion is not worthwhile as a routine technique in chemotherapeutic trials, for which the OCRC method is recommended.
采用盘尾丝虫病化疗研究中心的标准技术（OCRC方法）、盘尾丝虫病控制计划所采用的方法（OCP方法）以及胶原酶消化后，测定了100名患者400份皮肤切片中的微丝蚴密度。OCRC方法相当稳定，能检测出总微丝蚴的84%。OCP方法得出的密度始终比OCRC方法低20%，这表明皮肤切片在盐水中孵育后重量的增加相当可预测。得出的结论是，在化疗试验中，胶原酶消化作为一种常规技术不值得采用，化疗试验推荐使用OCRC方法。
