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J Struct Biol. 2010 Dec;172(3):253-60. doi: 10.1016/j.jsb.2010.06.023. Epub 2010 Jul 3.
A protocol to attain high-resolution single-particle reconstructions is presented. The protocol is the concatenation of two procedures: one to obtain an ab initio low-resolution reconstruction, the other to determine a fixed point of the consecutive applications of fast projection matching and 3D reconstruction. It is a reciprocal space formulation where the Fourier coefficients of the 3D scattering density are expressed in terms of symmetry adapted functions and the 2D particle images are represented by their Fourier-Bessel transforms. The new protocol shows advantages in terms of speed and accuracy when compared to other methods currently in use. We illustrate its performance as applied to high-resolution cryo-electron micrographs of rotavirus.
本文提出了一种获得高分辨率单颗粒重建的方案。该方案是两个步骤的串联：一个是获得从头开始的低分辨率重建，另一个是确定连续应用快速投影匹配和 3D 重建的固定点。这是一个倒易空间的表述，其中 3D 散射密度的傅里叶系数用对称适应函数表示，2D 粒子图像用它们的傅里叶-贝塞尔变换表示。与目前使用的其他方法相比，新方案在速度和准确性方面具有优势。我们将其应用于轮状病毒的高分辨率冷冻电镜显微照片来说明其性能。
