Auckland Bioengineering Institute, The University of Auckland, Private Bag 92019, Auckland, New Zealand.
J Struct Biol. 2011 Jan;173(1):110-6. doi: 10.1016/j.jsb.2010.06.020. Epub 2010 Jul 3.
Cardiac ventricular trabeculae are widely used in the study of cardiac muscle function, primarily because their myocytes are axially-aligned. However, their collagen content has not been rigorously determined. In particular, it is unknown whether the content of collagen differs between specimens originating from the left (LV) and right (RV) ventricles and whether, indeed, either corresponds to the collagen content of the ventricular walls themselves. In order to redress this deficit of knowledge, we have used the techniques of fluorescence confocal microscopy and environmental scanning electron microscopy to quantify the proportion of perimysial collagen comprising the cross-sectional area of trabeculae carneae. In trabeculae from both the RV and LV of adult rat hearts, collagen may occupy as little as 1% or as much as 100% of the cross-section. For specimens of dimensions typically used experimentally, there was no difference in average collagen content (6.03 ± 5.14%, n = 33) of preparations from the two ventricles.
心室内肌小梁被广泛应用于心肌功能的研究，主要是因为它们的心肌细胞呈轴向排列。然而，其胶原含量尚未得到严格确定。特别是，尚不清楚胶原的含量在来源于左心室（LV）和右心室（RV）的标本之间是否存在差异，以及实际上是否与心室壁本身的胶原含量相对应。为了弥补这一知识空白，我们使用荧光共聚焦显微镜和环境扫描电子显微镜技术来定量分析构成心肌小梁横截面积的肌周胶原的比例。在成年大鼠心脏的 RV 和 LV 心室内肌小梁中，胶原可能仅占横截面的 1%或高达 100%。对于通常在实验中使用的标本尺寸，来自两个心室的制备物的平均胶原含量（6.03 ± 5.14%，n = 33）没有差异。
