McGill University, Montreal, Quebec, Canada.
Anal Biochem. 2010 Nov 1;406(1):80-2. doi: 10.1016/j.ab.2010.06.038. Epub 2010 Jun 27.
Urinary trimethylamine (TMA) and its oxide (TMAOx) are measured separately and as a mixture using (15)N-labeled internal standards and direct infusion electrospray with a quadrupole time-of-flight (Q-ToF) instrument. TMA is quaternized with trideuteromethyl iodide to avoid inclusion of endogenous tetramethylammonium ion in the TMA measurement, whereas TMAOx is measured as the protonated molecule. Measurements reported as percentage TMA made with separate and combined samples agree within 6% of the measured values and demonstrate that both TMA and TMAOx can be measured simultaneously in a single analysis. Moreover, the analysis is simpler and less tedious and time-consuming than some earlier methods.
使用（15）N 标记的内标物和直接进样电喷雾技术，通过四极杆飞行时间（Q-ToF）仪器分别测量和混合测量尿中的三甲基胺（TMA）及其氧化物（TMAOx）。用氚代三甲碘甲烷季铵化 TMA，以避免内源性四甲基铵离子被包括在 TMA 测量中，而 TMAOx 则被测量为质子化分子。分别和合并样品测量的 TMA 百分率报告值在测量值的 6%以内，这表明 TMA 和 TMAOx 可以在单个分析中同时测量。此外，与一些早期方法相比，该分析更简单、不繁琐、耗时更短。
