REQUIMTE, Dep. Química, FCT-UNL, 2829-516 Caparica, Portugal.
Biochem Biophys Res Commun. 2010 Jul 23;398(2):199-204. doi: 10.1016/j.bbrc.2010.06.059. Epub 2010 Jun 17.
Conjugated linoleic acid (CLA) is a dietary fatty acid frequently used as a body fat reducing agent whose effects upon cell membranes and cellular function remain unknown. Obese Zucker rats were fed atherogenic diets containing saturated fats of vegetable or animal origin with or without 1% CLA, as a mixture of cis(c)9,trans(t)11 and t10,c12 isomers. Plasma membrane vesicles obtained from visceral adipose tissue were used to assess the effectiveness of dietary fat and CLA membrane incorporation and its outcome on fluidity and permeability to water and glycerol. A significant decrease in adipose membrane fluidity was correlated with the changes observed in permeability, which seem to be caused by the incorporation of the t10,c12 CLA isomer into membrane phospholipids. These results indicate that CLA supplementation in obese Zucker rats fed saturated and cholesterol rich diets reduces the fluidity and permeability of adipose membranes, therefore not supporting CLA as a body fat reducing agent through membrane fluidification in obese fat consumers.
共轭亚油酸（CLA）是一种常用的膳食脂肪酸，作为一种体脂减少剂，但其对细胞膜和细胞功能的影响尚不清楚。肥胖型 Zucker 大鼠喂食含饱和脂肪的动脉粥样硬化饮食，这些饱和脂肪来源于植物或动物源，同时添加或不添加 1% CLA，CLA 以顺式（c）9，反式（t）11 和反式（t）10，顺式（c）12 异构体混合物的形式存在。从内脏脂肪组织中获得质膜囊泡，用于评估膳食脂肪和 CLA 膜掺入的效果及其对水和甘油通透性和流动性的影响。脂肪膜流动性的显著降低与通透性观察到的变化相关，这似乎是由于 t10，c12 CLA 异构体掺入膜磷脂引起的。这些结果表明，在喂食富含饱和脂肪和胆固醇的饮食的肥胖型 Zucker 大鼠中补充 CLA 可降低脂肪膜的流动性和通透性，因此不支持 CLA 作为肥胖脂肪消费者通过膜流化解体脂肪减少剂。
