• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

槲皮素和表没食子儿没食子酸酯在体外对人单核血细胞的细胞膜具有剂量依赖性的硬化和超极化作用。

Quercetin and epigallocatechin gallate induce in vitro a dose-dependent stiffening and hyperpolarizing effect on the cell membrane of human mononuclear blood cells.

作者信息

Margina Denisa, Ilie Mihaela, Gradinaru Daniela

机构信息

Biochemistry Department, Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, 6, TraianVuia Street, Bucharest 020956, Romania.

Toxicology Department, Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, 6, TraianVuia Street, Bucharest 020956, Romania.

出版信息

Int J Mol Sci. 2012;13(4):4839-4859. doi: 10.3390/ijms13044839. Epub 2012 Apr 17.

DOI:10.3390/ijms13044839
PMID:22606013
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3344249/
Abstract

The bioactivity of polyphenols is closely linked to their ability to interact with biological membranes. The study evaluates the in vitro effect of quercetin and epigallocatechin on the membrane anisotropy and transmembrane potential of peripheral blood mononuclear cells (PBMCs) isolated from 26 type 2 diabetes mellitus patients compared to 25 age matched controls. The in vitro assays were analyzed in correlation with the biochemical and inflammatory profile of the subjects and with insulin resistance parameters (HOMA-IR, plasma resistin) as well. For type 2 diabetes patients, the increase of HOMA-IR and resistin concentration was associated with a significant decrease of the PBMCs membrane anisotropy. The two tested polyphenols induced a dose-dependent hyperpolarizing effect and stiffening of the cell membranes for all tested subjects. Physiological levels of quercetin and epigallocatechin gallate had the tendency to normalize the PBMCs membrane anisotropy of the cells isolated from diabetes patients, bringing it to the level of cells isolated from normoglycemic ones. Epigallocatechin gallate induced higher effects compared to quercetin on the membranes isolated from subjects with higher cardiovascular risk. The decrease of membrane fluidity and the hyperpolarizing effect could explain the cardiovascular protective action of the tested compounds.

摘要

多酚的生物活性与其与生物膜相互作用的能力密切相关。该研究评估了槲皮素和表没食子儿茶素对从26名2型糖尿病患者分离出的外周血单个核细胞（PBMC）的膜各向异性和跨膜电位的体外作用，并与25名年龄匹配的对照组进行了比较。体外试验与受试者的生化和炎症特征以及胰岛素抵抗参数（HOMA-IR、血浆抵抗素）进行了相关性分析。对于2型糖尿病患者，HOMA-IR和抵抗素浓度的增加与PBMC膜各向异性的显著降低相关。两种受试多酚对所有受试对象均诱导出剂量依赖性的超极化作用和细胞膜变硬。槲皮素和表没食子儿茶素的生理水平倾向于使从糖尿病患者分离出的细胞的PBMC膜各向异性正常化，使其达到从血糖正常者分离出的细胞的水平。与槲皮素相比，表没食子儿茶素对具有较高心血管风险的受试者的细胞膜诱导出更高的作用。膜流动性的降低和超极化作用可以解释受试化合物的心血管保护作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59e6/3344249/14bcc55a1c51/ijms-13-04839f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59e6/3344249/15e6b97a7c9d/ijms-13-04839f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59e6/3344249/f15318912ee4/ijms-13-04839f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59e6/3344249/835ebaf223d4/ijms-13-04839f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59e6/3344249/b2be9f4d50f3/ijms-13-04839f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59e6/3344249/bd1aab3064e2/ijms-13-04839f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59e6/3344249/235a15f714c2/ijms-13-04839f6a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59e6/3344249/14bcc55a1c51/ijms-13-04839f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59e6/3344249/15e6b97a7c9d/ijms-13-04839f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59e6/3344249/f15318912ee4/ijms-13-04839f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59e6/3344249/835ebaf223d4/ijms-13-04839f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59e6/3344249/b2be9f4d50f3/ijms-13-04839f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59e6/3344249/bd1aab3064e2/ijms-13-04839f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59e6/3344249/235a15f714c2/ijms-13-04839f6a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59e6/3344249/14bcc55a1c51/ijms-13-04839f7.jpg

相似文献

1
Quercetin and epigallocatechin gallate induce in vitro a dose-dependent stiffening and hyperpolarizing effect on the cell membrane of human mononuclear blood cells.槲皮素和表没食子儿没食子酸酯在体外对人单核血细胞的细胞膜具有剂量依赖性的硬化和超极化作用。
Int J Mol Sci. 2012;13(4):4839-4859. doi: 10.3390/ijms13044839. Epub 2012 Apr 17.
2
In vitro effects of prolonged exposure to quercetin and epigallocatechin gallate of the peripheral blood mononuclear cell membrane.外周血单个核细胞膜长时间暴露于槲皮素和表没食子儿茶素没食子酸酯的体外效应。
Cell Mol Biol Lett. 2014 Dec;19(4):542-60. doi: 10.2478/s11658-014-0211-7. Epub 2014 Oct 13.
3
Quercetin and epigallocatechin gallate effects on the cell membranes biophysical properties correlate with their antioxidant potential.槲皮素和表没食子儿没食子酸酯对细胞膜生物物理性质的影响与其抗氧化潜力相关。
Gen Physiol Biophys. 2012 Mar;31(1):47-55. doi: 10.4149/gpb_2012_005.
4
Membranar effects exerted in vitro by polyphenols - quercetin, epigallocatechin gallate and curcumin - on HUVEC and Jurkat cells, relevant for diabetes mellitus.多酚类物质（槲皮素、表没食子儿茶素没食子酸酯和姜黄素）在体外对人脐静脉内皮细胞（HUVEC）和人 Jurkat 细胞产生的膜效应，与糖尿病相关。
Food Chem Toxicol. 2013 Nov;61:86-93. doi: 10.1016/j.fct.2013.02.046. Epub 2013 Mar 4.
5
Quercetin and epigallocatechin-3-gallate effect on the anisotropy of model membranes with cholesterol.槲皮素和表没食子儿没食子酸酯对含胆固醇的模型膜各向异性的影响。
Food Chem Toxicol. 2013 Nov;61:94-100. doi: 10.1016/j.fct.2013.03.007. Epub 2013 Mar 20.
6
Zinc ionophore activity of quercetin and epigallocatechin-gallate: from Hepa 1-6 cells to a liposome model.槲皮素和表没食子儿茶素没食子酸酯的锌离子载体活性：从 Hepa 1-6 细胞到脂质体模型。
J Agric Food Chem. 2014 Aug 13;62(32):8085-93. doi: 10.1021/jf5014633. Epub 2014 Jul 31.
7
Quercetin, luteolin, and epigallocatechin gallate promote glucose disposal in adipocytes with regulation of AMP-activated kinase and/or sirtuin 1 activity.槲皮素、木犀草素和表没食子儿茶素没食子酸酯通过调节AMP激活的蛋白激酶和/或沉默信息调节因子1的活性促进脂肪细胞中的葡萄糖代谢。
Planta Med. 2014 Aug;80(12):993-1000. doi: 10.1055/s-0034-1382864. Epub 2014 Jul 24.
8
Differential protective effects of quercetin, resveratrol, rutin and epigallocatechin gallate against mitochondrial dysfunction induced by indomethacin in Caco-2 cells.槲皮素、白藜芦醇、芦丁和没食子儿茶素没食子酸酯对消炎痛诱导的 Caco-2 细胞线粒体功能障碍的差异保护作用。
Chem Biol Interact. 2012 Feb 5;195(3):199-205. doi: 10.1016/j.cbi.2011.12.007. Epub 2011 Dec 27.
9
Opposite effects of quercetin, luteolin, and epigallocatechin gallate on insulin sensitivity under normal and inflammatory conditions in mice.槲皮素、木犀草素和表没食子儿茶素没食子酸酯在正常和炎症条件下对小鼠胰岛素敏感性的相反作用。
Inflammation. 2013 Feb;36(1):1-14. doi: 10.1007/s10753-012-9514-x.
10
Green tea component, catechin, induces apoptosis of human malignant B cells via production of reactive oxygen species.绿茶成分儿茶素通过产生活性氧诱导人恶性B细胞凋亡。
Clin Cancer Res. 2005 Aug 15;11(16):6040-9. doi: 10.1158/1078-0432.CCR-04-2273.

引用本文的文献

1
Vitamin D and COVID-19: comparative analysis with other respiratory infections and impact of comorbidities.维生素D与2019冠状病毒病：与其他呼吸道感染的比较分析及合并症的影响
Germs. 2024 Sep 30;14(3):232-245. doi: 10.18683/germs.2024.1435. eCollection 2024 Sep.
2
Circadian Rhythms, Chrononutrition, Physical Training, and Redox Homeostasis-Molecular Mechanisms in Human Health.昼夜节律、时间营养、体育锻炼与氧化还原动态平衡——人类健康的分子机制
Cells. 2024 Jan 11;13(2):138. doi: 10.3390/cells13020138.
3
Intermittent Fasting on Human Health and Disease.

本文引用的文献

1
Peripheral blood mononuclear cell membrane fluidity and disease outcome in patients with multiple sclerosis.多发性硬化症患者外周血单个核细胞膜流动性与疾病转归
Indian J Hematol Blood Transfus. 2012 Mar;28(1):1-6. doi: 10.1007/s12288-011-0087-x. Epub 2011 Jun 21.
2
Fluidity modulation of phospholipid bilayers by electrolyte ions: insights from fluorescence microscopy and microslit electrokinetic experiments.电解质离子对磷脂双分子层流动性的调节：荧光显微镜和微缝电动实验的见解。
J Phys Chem A. 2012 Jun 28;116(25):6519-25. doi: 10.1021/jp212364q. Epub 2012 Feb 24.
3
Interactions between polyphenols and macromolecules: quantification methods and mechanisms.
间歇性禁食对人类健康和疾病的影响
Nutrients. 2023 Oct 24;15(21):4491. doi: 10.3390/nu15214491.
4
Zinc status, insulin resistance and glycoxidative stress in elderly subjects with type 2 diabetes mellitus.2型糖尿病老年患者的锌状态、胰岛素抵抗和糖氧化应激
Exp Ther Med. 2021 Dec;22(6):1393. doi: 10.3892/etm.2021.10829. Epub 2021 Sep 30.
5
Pharmacological Insights into Halophyte Bioactive Extract Action on Anti-Inflammatory, Pain Relief and Antibiotics-Type Mechanisms.盐生植物生物活性提取物在抗炎、止痛和抗生素类机制方面的药理学研究进展。
Molecules. 2021 May 24;26(11):3140. doi: 10.3390/molecules26113140.
6
Total accepted phenolic, tannin, triterpenoid, flavonoid and sterol contents, anti-diabetic, anti-inflammatory and cytotoxic activities of (Fee.) Sledge.（费氏）斯莱奇的总酚、单宁、三萜、黄酮和甾醇含量、抗糖尿病、抗炎和细胞毒性活性
Toxicol Rep. 2020 Oct 20;7:1465-1468. doi: 10.1016/j.toxrep.2020.10.013. eCollection 2020.
7
Analysis of the intricate effects of polyunsaturated fatty acids and polyphenols on inflammatory pathways in health and disease.多不饱和脂肪酸和多酚对健康与疾病中炎症通路的复杂影响分析
Food Chem Toxicol. 2020 Sep;143:111558. doi: 10.1016/j.fct.2020.111558. Epub 2020 Jul 5.
8
Chronic Inflammation in the Context of Everyday Life: Dietary Changes as Mitigating Factors.日常生活中的慢性炎症：饮食变化作为缓解因素。
Int J Environ Res Public Health. 2020 Jun 10;17(11):4135. doi: 10.3390/ijerph17114135.
9
Obesity ‑ a risk factor for increased COVID‑19 prevalence, severity and lethality (Review).肥胖——增加 COVID-19 患病率、严重程度和致死率的危险因素（综述）。
Mol Med Rep. 2020 Jul;22(1):9-19. doi: 10.3892/mmr.2020.11127. Epub 2020 May 5.
10
Biophysical Approach to Mechanisms of Cancer Prevention and Treatment with Green Tea Catechins.绿茶儿茶素预防和治疗癌症机制的生物物理方法
Molecules. 2016 Nov 18;21(11):1566. doi: 10.3390/molecules21111566.
多酚与大分子的相互作用：定量方法和机制。
Crit Rev Food Sci Nutr. 2012;52(3):213-48. doi: 10.1080/10408398.2010.499808.
4
Dietary oxidative stress and antioxidant defense with an emphasis on plant extract administration.饮食氧化应激与抗氧化防御——重点关注植物提取物的应用。
Cell Stress Chaperones. 2012 Jan;17(1):11-21. doi: 10.1007/s12192-011-0293-3. Epub 2011 Sep 30.
5
Basic biochemical mechanisms behind the health benefits of polyphenols.多酚对健康有益的基本生化机制。
Mol Aspects Med. 2010 Dec;31(6):435-45. doi: 10.1016/j.mam.2010.09.006. Epub 2010 Sep 18.
6
An inter-laboratory validation of methods of lipid peroxidation measurement in UVA-treated human plasma samples.经 UVA 处理的人血浆样本中脂质过氧化测量方法的实验室间验证。
Free Radic Res. 2010 Oct;44(10):1203-15. doi: 10.3109/10715762.2010.499907.
7
Conjugated linoleic acid reduces permeability and fluidity of adipose plasma membranes from obese Zucker rats.共轭亚油酸降低肥胖 Zucker 大鼠脂肪细胞膜的通透性和流动性。
Biochem Biophys Res Commun. 2010 Jul 23;398(2):199-204. doi: 10.1016/j.bbrc.2010.06.059. Epub 2010 Jun 17.
8
Hormesis and synergy: pathways and mechanisms of quercetin in cancer prevention and management.槲皮素在癌症预防和管理中的作用：协同与适应现象及机制。
Nutr Rev. 2010 Jul;68(7):418-28. doi: 10.1111/j.1753-4887.2010.00301.x.
9
Estrogen receptor profiles in human peripheral blood lymphocytes.人外周血淋巴细胞中雌激素受体谱。
Immunol Lett. 2010 Aug 16;132(1-2):79-85. doi: 10.1016/j.imlet.2010.06.003. Epub 2010 Jun 11.
10
(-)-Epigallocatechin gallate inhibits growth and activation of the VEGF/VEGFR axis in human colorectal cancer cells.(-)-表没食子儿茶素没食子酸酯抑制人结直肠癌细胞中 VEGF/VEGFR 轴的生长和激活。
Chem Biol Interact. 2010 May 14;185(3):247-52. doi: 10.1016/j.cbi.2010.03.036. Epub 2010 Mar 25.