Dipartimento di Biotecnologie e Bioscienze, Università di Milano-Bicocca, Piazza della Scienza 2, 20126 Milano, Italy.
Biochem Biophys Res Commun. 2010 Jul 16;398(1):44-50. doi: 10.1016/j.bbrc.2010.06.028. Epub 2010 Jun 17.
CK2 is a highly conserved protein kinase controlling different cellular processes. It shows a higher activity in proliferating mammalian cells, in various types of cancer cell lines and tumors. The findings presented herein provide the first evidence of an in vivo modulation of CK2 activity, dependent on growth rate, in Saccharomyces cerevisiae. In fact, CK2 activity, assayed on nuclear extracts, is shown to increase in exponential growing batch cultures at faster growth rate, while localization of catalytic and regulatory subunits is not nutritionally modulated. Differences in intracellular CK2 activity of glucose- and ethanol-grown cells appear to depend on both increase in molecule number and k(cat). Also in chemostat cultures nuclear CK2 activity is higher in faster growing cells providing the first unequivocal demonstration that growth rate itself can affect CK2 activity in a eukaryotic organism.
CK2 是一种高度保守的蛋白激酶，控制着不同的细胞过程。它在增殖的哺乳动物细胞、各种类型的癌细胞系和肿瘤中表现出更高的活性。本文提供的研究结果首次证明了在酿酒酵母中，CK2 活性依赖于生长速度而发生体内调节。事实上，在指数生长的分批培养物中，核提取物中 CK2 的活性随着生长速度的增加而增加，而催化亚基和调节亚基的定位不受营养调节。葡萄糖和乙醇生长的细胞中细胞内 CK2 活性的差异似乎取决于分子数量的增加和 k(cat)。在恒化培养物中，生长速度较快的细胞中核 CK2 的活性更高，这首次明确证明了生长速度本身可以影响真核生物中的 CK2 活性。
