Laboratory of Molecular Biology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Biochem Biophys Res Commun. 2010 Jul 30;398(3):547-52. doi: 10.1016/j.bbrc.2010.06.116. Epub 2010 Jul 1.
S100A9 is a member of the S100 family of proteins that contain two EF-hand calcium-binding motifs. We previously reported that S100A9 was differentially expressed during the early airway response phase of asthma and can be regulated by acupuncture. To understand the possible role of S100A9 in asthma, the effects of the S100A9 were investigated in a rat model of asthma and in isolated tracheal spirals. The pulmonary function and isometric tension were measured after the administration of purified recombinant S100A9. The results of in vivo experiments showed that S100A9 (0.1microg/kg) significantly decreased the pulmonary resistance and increased the dynamic compliance. The in vitro experimental results showed that S100A9 (100, 200, 400, or 800ng/ml, final concentrations) significantly reduced the isometric tension of isolated tracheal spirals. These results suggest that S100A9 elicits dose-dependent anti-asthmatic effects and may provide further insight into the treatment of asthma.
S100A9 是 S100 蛋白家族的一员，该家族蛋白含有两个 EF 手钙离子结合模体。我们之前的研究报道，S100A9 在哮喘的早期气道反应阶段呈差异表达，并且可以通过针灸进行调控。为了了解 S100A9 在哮喘中的可能作用，我们在哮喘大鼠模型和离体气管螺旋中研究了 S100A9 的作用。在给予纯化重组 S100A9 后，测量了肺功能和等长张力。体内实验结果表明，S100A9（0.1μg/kg）显著降低了肺阻力并增加了动态顺应性。体外实验结果表明，S100A9（100、200、400 或 800ng/ml，终浓度）显著降低了离体气管螺旋的等长张力。这些结果表明，S100A9 引起了剂量依赖性的抗哮喘作用，并且可能为哮喘的治疗提供了进一步的见解。
