Department of Clinical Biochemistry, University Hospital of South Manchester, UK.
Clin Biochem. 2011 Jan;44(1):110-8. doi: 10.1016/j.clinbiochem.2010.06.014. Epub 2010 Jul 1.
Assays using dried blood samples have been in use for a number of years particularly for the diagnosis of inborn errors of metabolism. This sampling technique has been greatly helped by the sensitivity and specificity offered by liquid chromatography tandem mass spectrometry because of the limited amount of sample available for analysis. There are advantages for patients to take samples at home but these must be weighed against potential problems with the sampling technique. Some assays appear to work very well whilst others suffer from poor recovery because of adsorption of the analyte onto the filter paper. Some newer strategies including impregnation of the filter paper and filter filtration will be discussed. Calibration of assays and the problems with matrix matching of standards are also important issues that need to be addressed before an assay can be used routinely.
干血斑样本检测法已经使用了很多年，特别是在诊断先天性代谢缺陷方面。由于可用于分析的样本量有限，液相色谱串联质谱的灵敏度和特异性极大地促进了这种采样技术的发展。患者在家中采样有优势，但必须权衡采样技术可能存在的问题。一些检测方法似乎效果很好，而另一些则因分析物被滤纸吸附而回收率差。本文将讨论一些新的策略，包括滤纸浸渍和过滤。在常规使用检测方法之前，还需要解决校准和标准基质匹配的问题。
