Laboratoire de Biochimie, UR 'Nutrition et Désordres Métaboliques' Faculté de médecine, Monastir, Tunisia.
Clin Biochem. 2010 Sep;43(13-14):1079-84. doi: 10.1016/j.clinbiochem.2010.06.010. Epub 2010 Jul 1.
To verify if HDL3 Anionic Peptide Factor (HDL3-APF) is as an apolipoprotein that promotes the reverse cholesterol transport.
We investigated a possible association between plasma HDL3-APF concentration, cholesterol efflux from Fu5AH cells and cholesteryl ester transfer protein (CETP) activity in type 2 diabetic patients with coronary artery disease (CAD) (n=36), those without CAD (n=20), and 37 healthy subjects.
Plasma APF concentrations were decreased in diabetics with CAD compared to controls (p<0.01). Cellular cholesterol efflux was decreased in diabetics without and with CAD, (p<0.01 and p<0.001 respectively). CETP activity was significantly elevated in all patient groups. Multiple linear regression analysis shows that cholesterol efflux was independently and positively related only to APF concentrations in controls.
APF is likely to be a key independent factor for promoting cellular cholesterol efflux in healthy subjects. However this association is altered in type 2 diabetes.
验证高密度脂蛋白 3 阴离子肽因子 (HDL3-APF) 是否为一种促进胆固醇逆向转运的载脂蛋白。
我们研究了 36 例伴有冠心病 (CAD) 的 2 型糖尿病患者、20 例不伴有 CAD 的糖尿病患者和 37 例健康对照者血浆 HDL3-APF 浓度、Fu5AH 细胞胆固醇流出率和胆固醇酯转移蛋白 (CETP) 活性之间可能存在的关联。
与对照组相比，伴有 CAD 的糖尿病患者的血浆 APF 浓度降低（p<0.01）。无 CAD 和伴有 CAD 的糖尿病患者的细胞胆固醇流出率降低（分别为 p<0.01 和 p<0.001）。所有患者组的 CETP 活性均显著升高。多元线性回归分析显示，仅在对照组中，胆固醇流出率与 APF 浓度呈独立正相关。
APF 可能是健康受试者促进细胞胆固醇流出的关键独立因素。然而，这种关联在 2 型糖尿病中发生了改变。
