Department of Neuroscience, University of Florida College of Medicine and McKnight Brain Institute, PO Box 100244, 100 S. Newell Dr., Gainesville, FL 32610, USA.
Exp Neurol. 2010 Sep;225(1):231-6. doi: 10.1016/j.expneurol.2010.06.006. Epub 2010 Jun 18.
Despite extensive gray matter loss following spinal cord injury (SCI), little attention has been given to neuronal replacement strategies and their effects on specific functional circuits in the injured spinal cord. In the present study, we assessed breathing behavior and phrenic nerve electrophysiological activity following transplantation of microdissected dorsal or ventral pieces of rat fetal spinal cord tissue (FSC(D) or FSC(V), respectively) into acute, cervical (C2) spinal hemisections. Transneuronal tracing demonstrated connectivity between donor neurons from both sources and the host phrenic circuitry. Phrenic nerve recordings revealed differential effects of dorsally vs. ventrally derived neural progenitors on ipsilateral phrenic nerve recovery and activity. These initial results suggest that local gray matter repair can influence motoneuron function in targeted circuits following spinal cord injury and that outcomes will be dependent on the properties and phenotypic fates of the donor cells employed.
尽管脊髓损伤 (SCI) 后会发生广泛的灰质损失，但人们对神经元替代策略及其对损伤脊髓中特定功能回路的影响关注甚少。在本研究中，我们评估了将微分离的大鼠胎脊髓组织（FSC(D) 或 FSC(V)）的背侧或腹侧片分别移植到急性颈（C2）脊髓半切后，呼吸行为和膈神经电生理活动的变化。转神经元示踪显示了来自两个来源的供体神经元与宿主膈神经回路之间的连接。膈神经记录显示背侧和腹侧来源的神经前体细胞对同侧膈神经恢复和活动有不同的影响。这些初步结果表明，局部灰质修复可以影响脊髓损伤后靶向回路中运动神经元的功能，并且结果将取决于所使用的供体细胞的特性和表型命运。
