College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
Exp Parasitol. 2011 Jan;127(1):42-5. doi: 10.1016/j.exppara.2010.06.034. Epub 2010 Jul 1.
Cryptosporidium and Giardia infections are common causes of diarrhea worldwide. To better understand the transmission of human cryptosporidiosis and giardiasis in Henan, China, 10 Cryptosporidium-positive specimens and 18 Giardia-positive specimens were characterized at the species/genotype and subtype levels. Cryptosporidium specimens were analyzed by DNA sequencing of the small subunit rRNA and 60kDa glycoprotein genes. Among those genotyped, nine belonged to C. hominis and one C. felis, with the former belonging to three subtype families: Ia, Ib, and Id. The three Ib subtypes identified, IbA16G2, IbA19G2, and IbA20G2, were very different from the two common Ib subtypes (IbA9G3 and IbA10G2) found in other areas of the world. The distribution of Giardia duodenalis genotypes and subtypes was assessed by sequence analysis of the triosephosphate isomerase (tpi) gene. The assemblages A (eight belonging to A-I and four A-II) and B (belonging to six new subtypes) were found in 12 and six specimens, respectively. More systematic studies are needed to understand the transmission of Cryptosporidium and G. duodenalis in humans in China.
隐孢子虫和贾第虫感染是全世界腹泻的常见原因。为了更好地了解中国河南地区人类隐孢子虫病和贾第虫病的传播情况，对 10 份隐孢子虫阳性标本和 18 份贾第虫阳性标本进行了种/基因型和亚型水平的特征分析。通过小亚基 rRNA 和 60kDa 糖蛋白基因的 DNA 测序分析隐孢子虫标本。在那些被分型的标本中，有九个属于人隐孢子虫，一个属于猫隐孢子虫，前者属于三个亚型家族：Ia、Ib 和 Id。鉴定出的三种 Ib 亚型，IbA16G2、IbA19G2 和 IbA20G2，与在世界其他地区发现的两种常见 Ib 亚型（IbA9G3 和 IbA10G2）有很大不同。通过三磷酸甘油异构酶（tpi）基因序列分析评估了十二指肠贾第鞭毛虫基因型和亚型的分布。发现了 12 个标本属于 A （8 个属于 A-I 和 4 个 A-II）和 6 个标本属于 B （属于 6 个新亚型）。需要进行更多的系统研究，以了解中国人类隐孢子虫和贾第鞭毛虫的传播情况。
