Subteam for Manipulation of Cell Fate, RIKEN BioResource Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan.
FEBS Lett. 2010 Aug 4;584(15):3402-9. doi: 10.1016/j.febslet.2010.06.036. Epub 2010 Jun 30.
Mitochondrial energy production is involved in various cellular processes. Here we show that ATP content is significantly increased in lineage-restricted progenitor cells compared with hematopoietic stem and progenitor cells (HSPCs) or more differentiated cells. Transplantation analysis using a mouse model of mitochondrial disease revealed that mitochondrial respiration defects resulted in a significant decrease in the total number and repopulating activity of bone marrow cells, although the number of HSPCs increased. The proliferative activity of HSPCs and lineage-restricted progenitor cells was not impaired by reduction of ATP content and there seems to be no associated increase in reactive oxygen species levels and apoptosis. Our findings indicate that mitochondrial respiration defects modulate HSPC commitment/differentiation into lineage-restricted progenitor cells.
线粒体的能量产生涉及多种细胞过程。在这里，我们发现与造血干/祖细胞（HSPCs）或更分化的细胞相比，谱系限制祖细胞中的 ATP 含量显著增加。使用线粒体疾病的小鼠模型进行的移植分析表明，线粒体呼吸缺陷导致骨髓细胞的总数和重编程活性显著减少，尽管 HSPCs 的数量增加。ATP 含量的减少并没有损害 HSPC 和谱系限制祖细胞的增殖活性，并且似乎没有相关的活性氧水平和细胞凋亡增加。我们的研究结果表明，线粒体呼吸缺陷调节 HSPC 向谱系限制祖细胞的定向分化。
