School of Agriculture, Food Science and Veterinary Medicine, UCD, Belfield, Dublin 4, Ireland.
Vaccine. 2010 Aug 2;28(34):5551-7. doi: 10.1016/j.vaccine.2010.06.039. Epub 2010 Jun 25.
The liver fluke, Fasciola hepatica causes liver fluke disease, or fasciolosis, in ruminants such as cattle and sheep. An effective vaccine against the helminth parasite is essential to reduce our reliance on anthelmintics, particularly in light of frequent reports of resistance to some frontline drugs. In our study, Friesian cattle (13 per group) were vaccinated with recombinant F. hepatica cathepsin L1 protease (rFhCL1) formulated in mineral-oil based adjuvants, Montanide ISA 70VG and ISA 206VG. Following vaccination the animals were exposed to fluke-contaminated pastures for 13 weeks. At slaughter, there was a significant reduction in fluke burden of 48.2% in the cattle in both vaccinated groups, relative to the control non-vaccinated group, at p<or=0.05. All vaccinated animals showed a sharp rise in total IgG levels to rFhCL1 post-vaccination which was maintained over the course of the 13-week challenge infection and was significantly higher than levels reached in the control group. Arginase levels in the macrophages of vaccinated cattle were significantly lower than those of the control cattle, indicating that the parasite-induced alternative-activation of the macrophages was altered by vaccination. The data demonstrate the potential for recombinant FhCL1 vaccine in controlling fasciolosis in cattle under field conditions.
肝片吸虫，又称双腔吸虫，可引起反刍动物（如牛、羊）肝片吸虫病或双腔吸虫病。针对这种寄生虫，开发有效的疫苗对于减少我们对驱虫药物的依赖至关重要，尤其是鉴于一些一线药物的耐药性问题时有发生。在我们的研究中，弗里斯兰牛（每组 13 头）接种了重组肝片形吸虫组织蛋白酶 L1 蛋白酶（rFhCL1），并使用矿物油佐剂 Montanide ISA 70VG 和 ISA 206VG 进行了配制。接种后，这些动物被暴露于受肝片吸虫污染的牧场上 13 周。在屠宰时，与未接种疫苗的对照组相比，两组接种疫苗的牛的肝片吸虫负荷量分别显著降低了 48.2%，p<0.05。所有接种疫苗的动物在接种后对 rFhCL1 的总 IgG 水平急剧上升，并且在 13 周的挑战感染过程中一直保持着这种高水平，显著高于对照组达到的水平。接种疫苗的牛的巨噬细胞中的精氨酸酶水平明显低于对照组的牛，表明疫苗接种改变了寄生虫诱导的巨噬细胞的替代性激活。这些数据表明，重组 FhCL1 疫苗具有在野外条件下控制牛双腔吸虫病的潜力。
