College of Life Sciences, Henan Normal University, Xinxiang, Henan 453007, China.
Food Chem Toxicol. 2010 Oct;48(10):2712-8. doi: 10.1016/j.fct.2010.06.045. Epub 2010 Jul 3.
The antioxidant and pro-oxidant potential of an extract from red radish, in which the major compounds were acylated pelargonidin derivatives, were assessed with a variety of assays in vitro. The extract appeared to form a complex with Fe(3+) or Cu(2+). It displayed a concentration-dependant reducing power (1.16OD(700 nm) at a concentration of 4mM) and scavenging effect against 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radicals (with IC(50)=1.74 +/- 0.03 mM). It could promote the cleavage of plasmid DNA with Cu(II)/H(2)O(2) or Cu(II) alone. This DNA damage could be inhibited by horseradish peroxidase, catalase, and EDTA, respectively. The extract also showed growth inhibition of Bel-7402 cells at lower concentration. The results suggested that the formation of reactive oxygen species might be involved in the mechanism of DNA damage. The acylated pelargonidin derivatives extracted from red radish could act as antioxidant and pro-oxidant and their antioxidant and pro-oxidant properties were relative to the reaction conditions. It might provide novel antioxidant and anticarcinogenic agents.
从红萝卜中提取的一种提取物，其主要化合物为酰化矢车菊素衍生物，具有抗氧化和促氧化潜力。通过各种体外试验评估了该提取物与 Fe(3+)或 Cu(2+)形成复合物的能力。它表现出浓度依赖性的还原能力（在 4mM 浓度下为 1.16OD(700nm)），并对 2,2'-偶氮双（3-乙基苯并噻唑啉-6-磺酸）自由基具有清除作用（IC(50)=1.74 +/- 0.03mM）。它可以促进 Cu(II)/H(2)O(2)或 Cu(II)单独作用下质粒 DNA 的断裂。这种 DNA 损伤可以分别被辣根过氧化物酶、过氧化氢酶和 EDTA 抑制。该提取物在较低浓度下还显示出对 Bel-7402 细胞的生长抑制作用。结果表明，活性氧的形成可能参与了 DNA 损伤的机制。从红萝卜中提取的酰化矢车菊素衍生物可作为抗氧化剂和促氧化剂，其抗氧化和促氧化特性与反应条件有关。它可能提供新的抗氧化和抗癌剂。
