Department of Pharmacognosy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, China.
J Ethnopharmacol. 2010 Aug 19;131(1):220-3. doi: 10.1016/j.jep.2010.06.024. Epub 2010 Jun 18.
To evaluate the potential expectorant and antitussive activity of a traditional Chinese medicine.
The water extract and four fractions of the aerial part of Reineckia carnea were orally administrated to coughing mice induced by ammonium hydroxide and mice injected with phenol red, respectively, to investigate their medification effect on coughing and mucus secretion.
90% Ethanol fraction significantly lengthened the latent period of cough and decreased cough frequency caused by ammonium hydroxide at the dose of 0.372 g/kg (p<0.05). Sixty percent ethanol fraction reduced the cough frequency as well as the mucus secretion from mouse tracheas obviously at the dose of 0.570 g/kg (p<0.05) by measuring the tracheal output of phenol red in mice. The medication effects in multiple doses of the active fractions were then performed and it has been proved that the 60% ethanol and 90% ethanol fraction were curatively effective on expectoration and coughing, respectively both at the high and middle dose, which supplied proofs for the further research on chemical constituents in both of two effective fractions.
评价一种中药的潜在祛痰镇咳作用。
分别给咳嗽的小鼠口服杠板归地上部分的水提物和四个馏分，以及给注射了苯酚红的小鼠口服，以研究它们对咳嗽和黏液分泌的调节作用。
90%乙醇馏分在 0.372g/kg 剂量下显著延长了由氨引起的咳嗽潜伏期，并降低了咳嗽频率（p<0.05）。60%乙醇馏分在 0.570g/kg 剂量下明显降低了咳嗽频率和小鼠气管中的黏液分泌（p<0.05），通过测量小鼠气管中苯酚红的排出量。然后进行了活性馏分的多次剂量给药实验，证明 60%乙醇和 90%乙醇馏分在高、中剂量下对祛痰和咳嗽均有治疗作用，为进一步研究这两个有效馏分中的化学成分提供了依据。
