Shang Xiaofei, Wang Dongsheng, Miao Xiaolou, Wang Yu, Zhang Jiyu, Wang Xuezhi, Zhang Yu, Pan Hu
Key Laboratory of New Animal Drug Project, Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Science, Lanzhou, 730050, People's Republic of China.
Department of Emergency, Lanzhou General Hospital of PLA, Lanzhou, 730050, People's Republic of China.
BMC Complement Altern Med. 2015 May 22;15:154. doi: 10.1186/s12906-015-0671-y.
As an important traditional Tibetan (veterinary) medicine, the flowers of Meconopsis punicea (family Papaveraceae) have been used to treat pain, fever, cough, inflammation, liver heat and lung heat of humans and animals by local people for thousands of years. In this paper, we aimed to investigate the antinociceptive and anti-tussive activities of the ethanol extract of M. punicea (EEM).
Firstly, HPLC was used to analyze the main constituents of the ethanol extract of M. punicea. In animal experiments, the acetic acid-induced writhing response test, hot plate test, barbiturate-induced sleeping time and formalin tests were used to evaluate the antinociceptive activity. Then, ammonia-induced coughing and sulfur dioxide-induced coughing tests in mice as well as the phenol red secretion in trachea test were used to investigate the anti-tussive activity of the extract. Finally, an acute toxicity study was carried out.
The results showed that alkaloids and flavonoids were the main compounds in the ethanol extract of M. punicea flowers. The extract at 125, 250 and 500 mg/kg had good antinociceptive and anti-tussive activities in mice with a dose-dependent manner.
These findings suggested that EEM has significant bioactivities, and the active components of M. punicea should be studied further.
作为一种重要的传统藏（兽）药，紫堇科植物红花绿绒蒿的花在当地已被人们用于治疗人和动物的疼痛、发热、咳嗽、炎症、肝热和肺热达数千年之久。在本文中，我们旨在研究红花绿绒蒿乙醇提取物（EEM）的抗伤害性感受和镇咳活性。
首先，采用高效液相色谱法分析红花绿绒蒿乙醇提取物的主要成分。在动物实验中，采用醋酸诱导扭体反应试验、热板试验、巴比妥酸盐诱导睡眠时间试验和福尔马林试验来评估抗伤害性感受活性。然后，采用小鼠氨水诱导咳嗽试验、二氧化硫诱导咳嗽试验以及气管酚红分泌试验来研究该提取物的镇咳活性。最后，进行急性毒性研究。
结果表明，生物碱和黄酮类化合物是红花绿绒蒿花乙醇提取物中的主要化合物。125、250和500mg/kg剂量的提取物在小鼠中具有良好的抗伤害性感受和镇咳活性，且呈剂量依赖性。
这些发现表明EEM具有显著的生物活性，红花绿绒蒿的活性成分应进一步研究。
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