Loo J C, Gallicano K D, McGilveray I J, Beaudoin N, Jindal S L
Bureau of Drug Research, Tunney's Pasture, Ottawa, Ontario, Canada.
Clin Biochem. 1991 Feb;24(1):49-53. doi: 10.1016/0009-9120(91)90189-l.
Cyclosporine in whole blood samples from renal and cardiac transplant recipients was measured by high-performance liquid chromatography (HPLC) and by CYCLO-Trac SP specific radioimmunoassay (RIA). The fresh samples assayed by RIA were remeasured in a second laboratory after storage at -20 degrees C for two to six months and good interlaboratory agreement was obtained on the 59 samples assayed (y = 0.926x + 14.8 micrograms/L, r = 0.982). The calibration curve for RIA was not influenced by fresh whole blood, hemolyzed whole blood or serum matrix from normal volunteers. However, comparison of the RIA results from patient samples with those from an HPLC procedure showed that RIA values averaged about double those measured by HPLC. The difference is attributable to differences between the blood matrix of transplant and nontransplant subjects, rather than exclusively to the apparent nonspecific nature of the antibody.
采用高效液相色谱法（HPLC）和CYCLO-Trac SP特异性放射免疫分析法（RIA）对肾移植和心脏移植受者全血样本中的环孢素进行测定。通过RIA分析的新鲜样本在-20℃储存两至六个月后，在第二个实验室重新测定，对测定的59个样本获得了良好的实验室间一致性（y = 0.926x + 14.8微克/升，r = 0.982）。RIA的校准曲线不受正常志愿者新鲜全血、溶血全血或血清基质的影响。然而，将患者样本的RIA结果与HPLC方法的结果进行比较发现，RIA值平均约为HPLC测定值的两倍。这种差异归因于移植和非移植受试者血液基质的差异，而不仅仅是抗体明显的非特异性性质。
