Department of Obstetrics and Gynaecology, Medical School, University of Thessalia, Larissa, Greece.
Reprod Sci. 2010 Sep;17(9):849-53. doi: 10.1177/1933719110372421. Epub 2010 Jul 2.
This prospective study was designed to evaluate and clarify further whether the position of the polar body (PB) in relation to injection site during intracytoplasmatic sperm injection (ICSI) has an impact on fertilization and developmental rates and consequently clinical pregnancy outcome. The study included 264 patients undergoing 306 ICSI cycles from September 2007 to January 2009 performed by the same practitioner. Of all oocytes retrieved, 1736 were in metaphase II (MII). From every woman reaching ovum pick up, all MII-collected oocytes were allocated to 1 of the 4 groups according to PB orientation. In group A, MII oocytes were injected with the PB at 6 o'clock, group B with the PB at 7 o'clock, group C with the PB at 11 o'clock, and a group D with the PB at 12 o'clock. A significantly higher proportion of fertilized oocytes were produced from oocytes that had been injected with the PB at 11 o'clock (79.2%) as compared to those at 6 o'clock (70.5%), 7 o'clock (64.4%), and 12 o'clock (68.8%). Furthermore, embryos derived from oocytes that were injected with the PB at 11 o'clock appeared to be of higher quality score than those of the other groups of oocytes. A higher clinical pregnancy rate (28.7%) was obtained after the transfer of embryos from oocytes that had been injected with the PB at 11 o'clock. Given the higher fertilization, developmental, and pregnancy rate in the 11 o'clock group, it is suggested that this may be the preferred position of the PB at ICSI.
本前瞻性研究旨在进一步评估和阐明卵母细胞极体（PB）在胞质内单精子注射（ICSI）注射部位的位置是否对受精率、胚胎发育率以及临床妊娠结局有影响。该研究纳入了 2007 年 9 月至 2009 年 1 月由同一位医生进行的 306 个 ICSI 周期的 264 名患者。在所有取出的卵母细胞中，有 1736 个处于中期 II 期（MII）。从每位取卵的女性中，根据 PB 方向将所有收集的 MII 卵母细胞分配到 4 个组中的 1 个组。在组 A 中，MII 卵母细胞的 PB 注射在 6 点，组 B 的 PB 注射在 7 点，组 C 的 PB 注射在 11 点，组 D 的 PB 注射在 12 点。与 6 点（70.5%）、7 点（64.4%）和 12 点（68.8%）相比，PB 注射在 11 点的卵母细胞产生的受精卵母细胞比例显著更高（79.2%）。此外，与其他组的卵母细胞相比，来自 PB 注射在 11 点的卵母细胞的胚胎质量评分似乎更高。从 PB 注射在 11 点的卵母细胞中转移胚胎后，获得了更高的临床妊娠率（28.7%）。鉴于 11 点组的受精率、胚胎发育率和妊娠率较高，建议这可能是 ICSI 中 PB 的首选位置。
