人、马和牛转铁蛋白的电泳特性分析

Electrophoretic characterization of human, equine and bovine transferrins.

Penhallow R C, Mason A B, Woodworth R C

Department of Biochemistry, University of Vermont College of Medicine, Burlington 05405.

Comp Biochem Physiol B. 1991;98(1):41-5. doi: 10.1016/0305-0491(91)90305-w.

Abstract

Human, bovine and equine transferrins have been characterized with respect to mol. wt, and behavior on urea-polyacrylamide gels, and isoelectric focussing gels. 2. As shown by SDS-polyacrylamide gel electrophoresis human transferrin has one major polypeptide whereas both bovine and equine transferrins have two polypeptides. 3. The transferrins show multiple banded patterns on urea-polyacrylamide and isoelectric focussing gels, particularly when iron saturated. The various forms are not resolved by neuraminidase treatment.

摘要

已对人、牛和马的转铁蛋白在分子量、尿素 - 聚丙烯酰胺凝胶及等电聚焦凝胶上的行为进行了表征。2. 如十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳所示，人转铁蛋白有一条主要多肽链，而牛和马的转铁蛋白都有两条多肽链。3. 转铁蛋白在尿素 - 聚丙烯酰胺凝胶和等电聚焦凝胶上呈现多条带的图谱，特别是当铁饱和时。用神经氨酸酶处理无法分辨出各种形式。