Erhardt G
Anim Genet. 1986;17(4):343-52. doi: 10.1111/j.1365-2052.1986.tb00727.x.
Isoelectric focusing with carrier ampholytes in ultrathin polyacrylamide gels and polyacrylamide gel electrophoresis in a discontinuous buffer system were used for the separation of sheep transferrin variants. For identification of the different iron-binding sites of transferrin a stepwise urea gradient, different degrees of iron saturation and double one-dimensional electrophoresis were used. Isoelectric focusing results in an increased resolution of the Fe0-transferrin, Fe1-transferrin and Fe2-transferrin region. At the level of Fe0-transferrin and Fe1-transferrin the variants I, A, G, B, C, D, M, E, Q, P can be identified. The method is especially suitable for genetic studies. For screening purposes up to 108 samples can be separated within one run in an ultrathin gel.
在超薄聚丙烯酰胺凝胶中使用载体两性电解质进行等电聚焦，并在不连续缓冲系统中进行聚丙烯酰胺凝胶电泳，用于分离绵羊转铁蛋白变体。为了鉴定转铁蛋白的不同铁结合位点，使用了逐步尿素梯度、不同程度的铁饱和度和双向一维电泳。等电聚焦提高了Fe0-转铁蛋白、Fe1-转铁蛋白和Fe2-转铁蛋白区域的分辨率。在Fe0-转铁蛋白和Fe1-转铁蛋白水平上，可以鉴定出变体I、A、G、B、C、D、M、E、Q、P。该方法特别适用于遗传学研究。为了进行筛选，在一次运行中，在超薄凝胶中最多可分离108个样品。
